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Overview
Product name
Anti-MBD3 antibody - Carboxyterminal end
See all MBD3 products (9) ...
Description
Rabbit polyclonal to MBD3 - Carboxyterminal end
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
A KLH conjugated synthetic peptide selected from the C-terminal region of human MBD3.
Positive control
Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue and transfected/ non-transfected 293 cell lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/500.Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/50 - 1/100.
Target
Function
Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
Sequence similarities
Contains 1 MBD (methyl-CpG-binding) domain.
Cellular localization
Nucleus. Nuclear, in discrete foci.
Target information above from: UniProt accessionO95983
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI181826 antibody
- AU019209 antibody
- MBD 3 antibody
see all
Database links
- Entrez Gene: 53615 Human
- Omim: 603573 Human
- SwissProt: O95983 Human
- Unigene: 178728 Human
Anti-MBD3 antibody - Carboxyterminal end images:
Western blot - MBD3 antibody - Carboxyterminal end (ab71777)
All lanes : Anti-MBD3 antibody - Carboxyterminal end (ab71777) at 1/100 dilution
Lane 1 : Non-transfected 293 cell lysates
Lane 2 : MBD3 transfected 293 cell lysates
Lysates/proteins at 2 µg per lane.
Predicted band size : 33 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MBD3 antibody - Carboxyterminal end (ab71777)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab71777 at a 1:50 dilution. A peroxidase-conjugated secondary antibody was then used, followed by DAB staining.
References for Anti-MBD3 antibody - Carboxyterminal end (ab71777)
ab71777 has not yet been referenced specifically in any publications.
Publishing research using ab71777? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"