MBD3 antibody - ChIP Grade (ab3755)

Product Name 

MBD3 antibody - ChIP Grade

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MBD3 - ChIP Grade

Immunogen 

Synthetic peptide: LARDGEAPLDKACAEDD, corresponding to amino acids 254-270 of Human MBD 3.
BLAST 'LARDGEAPLDKACAEDD' with or

Reacts with 

Hu, Ms, Rat

Predicted to react 
(species key)

Mamm
(Due to sequence homology)

Specificity 

This antibody recognises the two variants of MBD 3 at their C-terminus.

Tested applications 
(see key)

ChIP, ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ChIP: Recommended to use 20 ul per million cells.
IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 32.8 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

MDA-MB-231 cells.

Cellular localization 

Nucleus. Note: Nuclear, in discrete foci.

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding

Relevance 

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (Bhattacharya et al.). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (Hendrich et al.). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 has homology to bacterial base excision repair DNA N-glycosylases/lyases (Petronzelli et al.). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (Bader et al.).

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

serum with 0.02% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for MBD3 antibody - ChIP Grade images, references, products related to ab3755 and other tools.

MBD3 antibody - ChIP Grade images:

References for MBD3 antibody - ChIP Grade (ab3755)

  This product has been used in: (two most recent references)

Fu J et al. Promoter regulation of the visinin-like subfamily of neuronal calcium sensor proteins by nuclear respiratory factor-1. J Biol Chem 284:27577-86 (2009). ChIP; Human. PubMed: 19674972

Reese KJ et al. Maintenance of paternal methylation and repression of the imprinted H19 gene requires MBD3. PLoS Genet 3:e137 (2007). PubMed: 17708683

See all 4 publication references for this product.


If you publish research using ab3755 please let us know so that we can cite the reference on this datasheet.

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