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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab16057? Please let us know so that we can cite the reference in this datasheet
ab16057 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - MBD3 antibody (ab16057)
All lanes : Anti-MBD3 antibody (ab16057) at 0.11 µg/ml
Lane 1 : HeLa
Lane 2 : Hela nuclear
Lane 3 : A431
Lane 4 : HeLa with
Lane 5 : HeLa nuclear with
Lane 6 : A431 with
Secondary
Alexa Fluor Goat polyclonal anti-Rabbit IgG (700)
Predicted band size : 33 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa (possible cross reactivity, but this band is not blocked).
Immunocytochemistry/ Immunofluorescence - MBD3 antibody (ab16057)
ICC/IF image of ab16057 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab16057, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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