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Overview
Product name
Anti-MBD3 antibody
See all MBD3 products (9) ...
Description
Rabbit polyclonal to MBD3
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human MBD3.
(Peptide available as ab16210.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 0.1 - 1 µg/ml.Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa).
Target
Function
Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
Sequence similarities
Contains 1 MBD (methyl-CpG-binding) domain.
Cellular localization
Nucleus. Nuclear, in discrete foci.
Target information above from: UniProt accessionO95983
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI181826 antibody
- AU019209 antibody
- MBD 3 antibody
see all
Database links
- Entrez Gene: 53615 Human
- Entrez Gene: 17192 Mouse
- Entrez Gene: 362834 Rat
- Omim: 603573 Human
- SwissProt: O95983 Human
- SwissProt: Q9Z2D8 Mouse
- Unigene: 178728 Human
- Unigene: 7142 Mouse
see all
Anti-MBD3 antibody images:
Western blot - MBD3 antibody (ab16057)
All lanes : Anti-MBD3 antibody (ab16057) at 0.11 µg/ml
Lane 1 : HeLa
Lane 2 : Hela nuclear
Lane 3 : A431
Lane 4 : HeLa with MBD3 peptide (ab16210) at 1 µg/ml
Lane 5 : HeLa nuclear with MBD3 peptide (ab16210) at 1 µg
Lane 6 : A431 with MBD3 peptide (ab16210) at 1 µg/ml
Secondary
Alexa Fluor Goat polyclonal anti-Rabbit IgG (700)
Predicted band size : 33 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa (possible cross reactivity, but this band is not blocked).
Immunocytochemistry/ Immunofluorescence - MBD3 antibody (ab16057)
ICC/IF image of ab16057 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab16057, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-MBD3 antibody (ab16057)
ab16057 has not yet been referenced specifically in any publications.
Publishing research using ab16057? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"