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- MBD3 antibody (ab16057)
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Immunocytochemistry/ Immunofluorescence - MBD3 antibody (ab16057)
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Product Name
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MBD3 antibody
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See all MBD3 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MBD3
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human MBD3.
(Peptides available as ab16209 and ab16210.)
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Reacts with
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Hu
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Predicted to react
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Ms, Rat
(Due to sequence homology)
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Tested applications
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ICC/IF, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nucleus. Note: Nuclear, in discrete foci.
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Research areas
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Chromatin and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding
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Relevance
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DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (Bhattacharya et al.). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (Hendrich et al.). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 has homology to bacterial base excision repair DNA N-glycosylases/lyases (Petronzelli et al.). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (Bader et al.).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MBD3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MBD3 antibody images, references, products related to ab16057 and other tools.
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MBD3 antibody images:
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 Immunocytochemistry/ Immunofluorescence - MBD3 antibody (ab16057)
ICC/IF image of ab16057 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab16057, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Search PubMed (MEDLINE) for references to MBD3
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MBD3 antibody - more information
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Customer reviews (feedback) regarding MBD3 antibody |
Customer FAQs regarding MBD3 antibody |
Protocols for MBD3 antibody |
Price and availability of products related to MBD3 antibody |
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MBD3 antibody - related products:
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MBD3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MBD3 antibody |
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