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Western blot - MBD3 antibody (ab69887)
Western blot - MBD3 antibody (ab69887)
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Product Name 

MBD3 antibody

 

See all MBD3 antibodies (8)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MBD3

Immunogen 

Synthetic peptide derived from the C-terminal of human MBD3.

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

ELISA, WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Extracts from Jurkat cells.

Cellular localization 

Nucleus. Note: Nuclear, in discrete foci.

Research areas 

Chromatin and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding

Relevance 

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (Bhattacharya et al.). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (Hendrich et al.). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 has homology to bacterial base excision repair DNA N-glycosylases/lyases (Petronzelli et al.). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (Bader et al.).

 

Alternative names MBD3 antibody (ab69887)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

53615    (Human)
17192    (Mouse)

Omim

    

603573    (Human)

SwissProt

    

O95983    (Human)
Q9Z2D8    (Mouse)

Unigene

    

178728    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MBD3 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for MBD3 antibody images, references, products related to ab69887 and other tools.

 

MBD3 antibody images:

Product image  Western blot - MBD3 antibody (ab69887)

Western blot - MBD3 antibody (ab69887)

All lanes : MBD3 antibody (ab69887) at 1/500 dilution

Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg

Lysates/proteins at 5 µg per lane.


Predicted band size : 33 kDa
Observed band size : 33 kDa

 
 

Search PubMed (MEDLINE) for references to MBD3

 

MBD3 antibody - more information

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Price and availability of products related to MBD3 antibody

 

MBD3 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for MBD3 antibody

ab14844

  

Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate

 

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MBD3 antibody - other tools:

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