Overview
- Product nameAnti-MBD3 antibodySee all MBD3 primary antibodies ...
- DescriptionMouse polyclonal to MBD3
- Tested applicationsWB more details
- Species reactivityReacts with: Human
- Immunogen
Full length protein corresponding to amino acids 1-291 of Human MBD3 (NP_003917.1).
- Positive control293 cell lysate, MBD3 transfected 293T cell lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab89440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. |
Target
- FunctionDoes not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
- Sequence similaritiesContains 1 MBD (methyl-CpG-binding) domain.
- Cellular localizationNucleus. Nuclear, in discrete foci.
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Database links
- Entrez Gene: 53615 Human
- Omim: 603573 Human
- SwissProt: O95983 Human
- Unigene: 178728 Human
Target information above from: UniProt accession
O95983
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- AI181826 antibodyAU019209 antibodyMBD 3 antibody
- Mbd3 antibodyMBD3: methyl CpG binding domain protein 3 antibodyMBD3_HUMAN antibodyMethyl CpG binding domain protein 3 antibodyMethyl CpG binding protein MBD3 antibodyMethyl-CpG-binding domain protein 3 antibodyMethyl-CpG-binding protein MBD3 antibody
see all
Anti-MBD3 antibody images
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Western blot - MBD3 antibody (ab89440)Anti-MBD3 antibody (ab89440) at 1 µg/ml + 293 cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 33 kDa
Observed band size : 33 kDa -
Western blot - MBD3 antibody (ab89440)All lanes : Anti-MBD3 antibody (ab89440) at 1 µg/ml
Lane 1 : MBD3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
developed using the ECL technique
Predicted band size : 33 kDa
Observed band size : 33 kDa
References for Anti-MBD3 antibody (ab89440)
ab89440 has not yet been referenced specifically in any publications.
