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Overview

  • Product nameMBD3 protein (Tagged-His Tag)See all MBD3 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 164-291 (128 amino acids) of Human MBD3 with N terminal His tag; Predicted MWt 15 kDa (UniProt O95983).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 86 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesGLQGVGPGCTDETLLSAIASALHTSTMPITGQLSAAVEKN PGVWLNTTQ PLCKAFMVTDEDIRKQEELVQQVRKRLEE ALMADMLAHVEELARDGEAP LDKACAEDDDEEDEEEEE EEPDPDPEMEHV
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91820 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        AI181826AU019209MBD 3
        Mbd3MBD3: methyl CpG binding domain protein 3MBD3_HUMANMethyl CpG binding domain protein 3Methyl CpG binding protein MBD3Methyl-CpG-binding domain protein 3Methyl-CpG-binding protein MBD3
      see all
  • FunctionDoes not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
  • Sequence similaritiesContains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localizationNucleus. Nuclear, in discrete foci.

    • Target information above from: UniProt accession O95983 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    MBD3 protein (Tagged-His Tag) images

    • SDS-PAGE - MBD3 protein (Tagged-His Tag) (ab91820)
      SDS-PAGE - MBD3 protein (Tagged-His Tag) (ab91820)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for MBD3 protein (Tagged-His Tag) (ab91820)

    ab91820 has not yet been referenced specifically in any publications.

    Publishing research using ab91820 ? Please let us know so that we can cite the reference in this datasheet

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