Anti-MBD4/MED1 antibody - C-terminal (ab191037)
Key features and details
- Rabbit polyclonal to MBD4/MED1 - C-terminal
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MBD4/MED1 antibody - C-terminal
See all MBD4/MED1 primary antibodies -
Description
Rabbit polyclonal to MBD4/MED1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human MBD4/MED1 aa 550 to the C-terminus (C terminal).
Database link: O95243 -
Positive control
- Rat brain and kidney tissue lysates; A549, HeLa and MCF-7 cell lysates; Recombinant Human MBD4/MED1 protein (aa400-580).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 66 kDa.
The detection limit for MBD4 is approximately 1ng/lane under reducing conditions. |
Notes |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 66 kDa. The detection limit for MBD4 is approximately 1ng/lane under reducing conditions. |
Target
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Function
Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. -
Sequence similarities
Contains 1 MBD (methyl-CpG-binding) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8930 Human
- Entrez Gene: 17193 Mouse
- Entrez Gene: 680915 Rat
- Omim: 603574 Human
- SwissProt: O95243 Human
- SwissProt: Q9Z2D7 Mouse
- Unigene: 35947 Human
- Unigene: 259308 Mouse
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Alternative names
- 3 N(4) ethenocytosine glycosylase antibody
- G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
- G/T mismatch glycosylase antibody
see all
Images
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All lanes : Anti-MBD4/MED1 antibody - C-terminal (ab191037) at 0.5 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : HeLa cell lysate
Predicted band size: 66 kDa -
All lanes : Anti-MBD4/MED1 antibody - C-terminal (ab191037) at 0.5 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat kidney tissue lysate
Lane 3 : A549 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : MCF-7 cell lysate
Predicted band size: 66 kDa -
All lanes : Anti-MBD4/MED1 antibody - C-terminal (ab191037) at 0.5 µg/ml
Lane 1 : Recombinant Human MBD4/MED1 protein (aa400-580) at 0.01 µg
Lane 2 : Recombinant Human MBD4/MED1 protein (aa400-580) at 0.005 µg
Lane 3 : Recombinant Human MBD4/MED1 protein (aa400-580) at 0.0025 µg
Predicted band size: 66 kDaE.coli derived -recombinant Human MBD4/MED1, 39.7 kDa (162aa tag + Q400-S580).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab191037 has not yet been referenced specifically in any publications.