MBD4 antibody (ab12187)

Product Name 

MBD4 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MBD4

Immunogen 

Synthetic peptide corresponding to amino acids 541 to 554 of mouse MBD4 conjugated to KLH via an N terminal added cysteine residue. The sequence is conserved in human.
BLAST 'GNDSYRIFCVNEWK' with or

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Chk
(Due to sequence homology)

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 70 kDa). Staining of the MBD4 band in immunoblotting is specifically inhibited by the immunizing peptide.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Nuclear extracts of K562 cell line.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding

Relevance 

Methyl CpG binding domain proteins (MBDs) are a family of mammalian proteins that specifically bind methylated regions of DNA. There are two methyl CpG binding complexes, MeCP1 and MeCP2. MeCP2 is comprised of a single protein also denoted MeCP2. MeCP1 is composed of four different proteins termed MBD1 (also known as PCM1), MBD2, MBD3, and MBD4 (also known as MED1).

MBD4 is involved in DNA repair. It has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. It can also remove uracil or 5-fluorouracil in G:U mismatches. It hasno lyase activity and was first identified as methyl-CpG-binding protein.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4.

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified using the immunizing peptide immobilized on agarose.

Form 

Liquid

Concentration 

1.300 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Storage in frost free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.

References for MBD4 antibody (ab12187)

  This product has been used in: (two most recent references)

Bertoni C et al. Enhanced gene repair mediated by methyl-CpG-modified single-stranded oligonucleotides. Nucleic Acids Res 37:7468-82 (2009). WB; Mouse. PubMed: 19854937

Hardeland U et al. Cell cycle regulation as a mechanism for functional separation of the apparently redundant uracil DNA glycosylases TDG and UNG2. Nucleic Acids Res 35:3859-67 (2007). PubMed: 17526518
If you publish research using ab12187 please let us know so that we can cite the reference on this datasheet.

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