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Overview
Product name
Anti-MBD4 antibody
See all MBD4 products (4) ...
Description
Rabbit polyclonal to MBD4
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Chicken
Immunogen
Synthetic peptide corresponding to amino acids 541 to 554 of mouse MBD4 conjugated to KLH via an N terminal added cysteine residue. The sequence is conserved in human.
GNDSYRIFCV NEWK
Positive control
Nuclear extracts of K562 cell line.
General notes
Storage in frost free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified using the immunizing peptide immobilized on agarose.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 70 kDa). Staining of the MBD4 band in immunoblotting is specifically inhibited by the immunizing peptide.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
Sequence similarities
Contains 1 MBD (methyl-CpG-binding) domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO95243
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 3 N(4) ethenocytosine glycosylase antibody
- G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
- G/T mismatch glycosylase antibody
see all
Database links
- Entrez Gene: 8930 Human
- Entrez Gene: 17193 Mouse
- Omim: 603574 Human
- SwissProt: O95243 Human
- SwissProt: Q8R3R3 Mouse
- SwissProt: Q9Z2D7 Mouse
- Unigene: 35947 Human
- Unigene: 259308 Mouse
References for Anti-MBD4 antibody (ab12187)
This product has been referenced in:
- Pettersen HSet al. UNG-initiated base excision repair is the major repair route for 5-fluorouracil in DNA, but 5-fluorouracil cytotoxicity depends mainly on RNA incorporation. Nucleic Acids Res : (2011). WB; Human.Read more (PubMed: 21745813) »
- Bertoni Cet al. Enhanced gene repair mediated by methyl-CpG-modified single-stranded oligonucleotides. Nucleic Acids Res 37:7468-82 (2009). WB; Mouse.Read more (PubMed: 19854937) »
See all 3 publications for this product
Publishing research using ab12187? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"