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Anti-MBD4 antibody (ab13793)

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Overview

Product name

Anti-MBD4 antibody
See all MBD4 products (4) ...

Description

Rabbit polyclonal to MBD4

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 268-282 (CNKADAESEPVAQKS) and 337-352 (CSAKDSEHNEKYEDTF) of human MBD4.

CNKADAESEP VAQKS

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HL-60 cell lysate.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

This antibody is affinity purified.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding

  • Western blot - Anti-MBD4 antibody (ab13793)Western blot - Anti-MBD4 antibody (ab13793) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab13793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/200 - 1/1000.Detec...Read more →

    WB: 1/200 - 1/1000.Detects a band of approximately 64 kDa (predicted molecular weight: 70 kDa).

Target

Function

Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.

Sequence similarities

Contains 1 MBD (methyl-CpG-binding) domain.

Cellular localization

Nucleus.

Target information above from: UniProt accessionO95243 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 3 N(4) ethenocytosine glycosylase antibody
  • G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
  • G/T mismatch glycosylase antibody
  • G/U mismatch glycosylase antibody
  • MBD 4 antibody
  • MBD4 antibody
  • MBD4_HUMAN antibody
  • MED 1 antibody
  • MED1 antibody
  • Methyl CpG binding domain protein 4 antibody
  • Methyl CpG binding endonuclease 1 antibody
  • Methyl CpG binding protein MBD4 antibody
  • Methyl-CpG-binding domain protein 4 antibody
  • Methyl-CpG-binding endonuclease 1 antibody
  • Methyl-CpG-binding protein MBD4 antibody
  • Mismatch specific DNA N glycosylase antibody
  • Mismatch-specific DNA N-glycosylase antibody
see all

Database links

Anti-MBD4 antibody images:

  Western blot - Anti-MBD4 antibody (ab13793)

Western blot - Anti-MBD4 antibody (ab13793)



Predicted band size : 70 kDa


ab13793, at 2 ug/ml, staining approximately 64 kDa human MBD4 in HL-60 cell lysate by Western blot.

References for Anti-MBD4 antibody (ab13793)

ab13793 has not yet been referenced specifically in any publications.

Publishing research using ab13793? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"