Overview

  • Product nameAnti-MBD4 antibody
    See all MBD4 primary antibodies
  • Description
    Rabbit polyclonal to MBD4
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 268-282 (

    CNKADAESEPVAQKS

    ) and 337-352 (CSAKDSEHNEKYEDTF) of human MBD4.

  • Positive control
    • HL-60 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is affinity purified.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab13793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 70 kDa).

Target

  • FunctionMismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
  • Sequence similaritiesContains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 N(4) ethenocytosine glycosylase antibody
    • G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
    • G/T mismatch glycosylase antibody
    • G/U mismatch glycosylase antibody
    • MBD 4 antibody
    • MBD4 antibody
    • MBD4_HUMAN antibody
    • MED 1 antibody
    • MED1 antibody
    • Methyl CpG binding domain protein 4 antibody
    • Methyl CpG binding endonuclease 1 antibody
    • Methyl CpG binding protein MBD4 antibody
    • Methyl-CpG-binding domain protein 4 antibody
    • Methyl-CpG-binding endonuclease 1 antibody
    • Methyl-CpG-binding protein MBD4 antibody
    • Mismatch specific DNA N glycosylase antibody
    • Mismatch-specific DNA N-glycosylase antibody
    • Putative methyl CpG binding protein antibody
    see all

Anti-MBD4 antibody images



  • Predicted band size : 70 kDa


    ab13793, at 2 ug/ml, staining approximately 64 kDa human MBD4 in HL-60 cell lysate by Western blot.

References for Anti-MBD4 antibody (ab13793)

ab13793 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for answering my questions. I'm sorry to hear you are having a problem with ab13793. I would like to suggest the following modifications to your protocol: Please try blocking with only 5% milk in TBST. 10% seems high to me, ...

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Thank you for your enquiry. Could you please tell me how you blocked the membrane? Also, did you add blocking agent to your primary antibody? I hope this information helps, please do not hesitate to contact us if you need any more advice or info...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"