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Overview

  • Product nameAnti-MBD4 antibodySee all MBD4 primary antibodies ...
  • Description
    Rabbit polyclonal to MBD4
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 268-282 (CNKADAESEPVAQKS) and 337-352 (CSAKDSEHNEKYEDTF) of human MBD4.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHL-60 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is affinity purified.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab13793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/200 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 70 kDa).

Target

  • FunctionMismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
  • Sequence similaritiesContains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession O95243 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        3 N(4) ethenocytosine glycosylase antibodyG/5 fluorouracil mismatch glycosylase with biphasic kinetics antibodyG/T mismatch glycosylase antibody
        G/U mismatch glycosylase antibodyMBD 4 antibodyMBD4 antibodyMBD4_HUMAN antibodyMED 1 antibodyMED1 antibodyMethyl CpG binding domain protein 4 antibodyMethyl CpG binding endonuclease 1 antibodyMethyl CpG binding protein MBD4 antibodyMethyl-CpG-binding domain protein 4 antibodyMethyl-CpG-binding endonuclease 1 antibodyMethyl-CpG-binding protein MBD4 antibodyMismatch specific DNA N glycosylase antibodyMismatch-specific DNA N-glycosylase antibody
      see all

    Anti-MBD4 antibody images

    • Western blot - Anti-MBD4 antibody (ab13793)
      Western blot - Anti-MBD4 antibody (ab13793)


      Predicted band size : 70 kDa


      ab13793, at 2 ug/ml, staining approximately 64 kDa human MBD4 in HL-60 cell lysate by Western blot.

    References for Anti-MBD4 antibody (ab13793)

    ab13793 has not yet been referenced specifically in any publications.

    Publishing research using ab13793 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab13793 Submit a Question for ab13793

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    Blocking with 10 % nonfat dry milk in TBST 1 hour at RT. Primary antibody diluted in 3% nonfat dry milk in TBST

    Thank you very much for answering my questions. I'm sorry to hear you are having a problem with ab13793. I would like to suggest the following modifications to your protocol: Please try blocking with only 5% milk in TBST. 10% seems high to me, you...

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    BATCH NUMBER 75947 ORDER NUMBER 64848 DESCRIPTION OF THE PROBLEM No band at all SAMPLE K562 cell extract PRIMARY ANTIBODY anti-MBD4 (ab13793), 1/100 and 1/200 dilution in TBST, overnight 10 min three time wash with TBST DETE...

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    Thank you for your enquiry. Could you please tell me how you blocked the membrane? Also, did you add blocking agent to your primary antibody? I hope this information helps, please do not hesitate to contact us if you need any more advice or informa...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"