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Overview
Product name
Anti-MBD4 antibody
See all MBD4 products (4) ...
Description
Rabbit polyclonal to MBD4
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide, corresponding to a region between residue 1 and 50 of Human MBD4 (NP_003916.1)
Positive control
Human Breast Carcinoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma and Testicular Seminoma tissues.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab84754 was affinity purified using an epitope specific to MBD4 immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/500.Antigen retrieval is not essential but may optimise staining.(The use of citrate buffer will enhance staining.)
Target
Function
Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
Sequence similarities
Contains 1 MBD (methyl-CpG-binding) domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO95243
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 3 N(4) ethenocytosine glycosylase antibody
- G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
- G/T mismatch glycosylase antibody
see all
Database links
- Entrez Gene: 8930 Human
- Omim: 603574 Human
- SwissProt: O95243 Human
- Unigene: 35947 Human
Anti-MBD4 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MBD4 antibody (ab84754)
ab84754 at 1/250 dilution staining MBD4 in Human testicular seminoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MBD4 antibody (ab84754)
ab84754 at 1/250 dilution staining MBD4 in Human ovarian tumor by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
References for Anti-MBD4 antibody (ab84754)
ab84754 has not yet been referenced specifically in any publications.
Publishing research using ab84754? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"