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This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
Overview
Product name
Anti-MBD6 antibody
Description
Rabbit polyclonal to MBD6
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Cross reactivity
Predicted to work with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human MBD6.
(Peptide available as ab38405.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Immunocytochemistry/ Immunofluorescence - MBD6 antibody (ab37926)
(enlarge)
Applications
Application notes
This antibody gave a positive result in ELISA against the immunizing peptide (ab38405).
Not yet tested in other applications.
Target
Relevance
MBD6 contains a methyl-CpG-binding domain (MBD) but it's function is currently unknown. DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) were first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 has homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
Cellular localization
Nuclear
Alternative names
- Methyl-CpG-binding domain protein 6 antibody
- Methyl-CpG-binding protein MBD6 antibody
Database links
- Entrez Gene: 114785 Human
- Entrez Gene: 110962 Mouse
- SwissProt: Q6P0P0 Human
- SwissProt: Q96DN6 Human
- SwissProt: Q3TY92 Mouse
- SwissProt: Q5XJH3 Mouse
- SwissProt: Q9D374 Mouse
- Unigene: 524523 Human
see all
Anti-MBD6 antibody images:
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Immunocytochemistry/ Immunofluorescence - MBD6 antibody (ab37926)
ICC/IF image of ab37926 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab37926, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293, HepG2 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
References for Anti-MBD6 antibody (ab37926)
ab37926 has not yet been referenced specifically in any publications.
Publishing research using ab37926? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"