Anti-MCAF1 antibody (ab84497)
Key features and details
- Rabbit polyclonal to MCAF1
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MCAF1 antibody -
Description
Rabbit polyclonal to MCAF1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide within Human MCAF1 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. AAQ92978.1
Database link: Q6VMQ6 -
Positive control
- Human small cell lung cancer. Mouse squamous cell carcinoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500.
Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. |
Notes |
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IHC-P
1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. |
Target
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Relevance
MCAF1 is a recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. It can act both as an activator or a repressor depending on the context. It mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. MCAF1 is required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9'. The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 55729 Human
- Entrez Gene: 54343 Mouse
- SwissProt: Q6VMQ6 Human
- SwissProt: Q7TT18 Mouse
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Alternative names
- Activating transcription factor 7 interacting protein 1 antibody
- ATF interacting protein antibody
- ATF IP antibody
see all
Images
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ab84497, at 1/250 dilution, staining MCAF1 in formalin-fixed, paraffin-embedded human small cell lung cancer by Immunohistochemistry, using DAB staining.
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ab84497, at 1/250 dilution, staining MCAF1 in formalin-fixed, paraffin-embedded mouse squamous cell carcinoma by Immunohistochemistry, using DAB staining.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab84497 has been referenced in 5 publications.
- Tsusaka T et al. The fibronectin type-III (FNIII) domain of ATF7IP contributes to efficient transcriptional silencing mediated by the SETDB1 complex. Epigenetics Chromatin 13:52 (2020). PubMed: 33256805
- Sin JH et al. The epigenetic regulator ATF7ip inhibits Il2 expression, regulating Th17 responses. J Exp Med 216:2024-2037 (2019). PubMed: 31217192
- Tsusaka T et al. ATF7IP regulates SETDB1 nuclear localization and increases its ubiquitination. EMBO Rep 20:e48297 (2019). PubMed: 31576654
- Tsusaka T et al. Tri-methylation of ATF7IP by G9a/GLP recruits the chromodomain protein MPP8. Epigenetics Chromatin 11:56 (2018). PubMed: 30286792
- Minkovsky A et al. The Mbd1-Atf7ip-Setdb1 pathway contributes to the maintenance of X chromosome inactivation. Epigenetics Chromatin 7:12 (2014). Mouse . PubMed: 25028596