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MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate - H2O2 post treated (ab14863)

Q&A (3)

Overview

  • Product nameMCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate - H2O2 post treatedSee all MCF7 lysates ...
  • General notesPost-collection treatment consists in exposing the nuclear extract to a final concentration of 200 mM H2O2 for 20 min on ice. The protein content has been determined by a Bradford-based assay.

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, Protease inhibitor, 400mM Sodium chloride, 20mM HEPES, 10mM Sodium fluoride, 10mM Para nitrophenyl phosphate, 10mM Beta glycerophosphate, 1mM DTT, 1mM Sodium metavanadate, 0.1mM EDTA, 0.01mM Sodium molybdate. pH 7.5
  • Concentration information loading...
  • Research Areas
  • BackgroundMCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.

    • MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate - H2O2 post treated images

    • MCF-7 Nuclear Extract H2O2 Post Treated
      MCF-7 Nuclear Extract H2O2 Post Treated

      Each lot has been tested for Sp1 activity by using the TransAM™ Sp1 Kit. The signal intensity for Sp1 activity in each lot is compared to the signal intensity obtained with H2O2-post treated extracts from MCF-7 cells. Once the signals are blanked, the ratio between the signals from untreated extracts over treated extracts used at 5 µg/well must be above 4.

    References for MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate - H2O2 post treated (ab14863)

    ab14863 has not yet been referenced specifically in any publications.

    Publishing research using ab14863 ? Please let us know so that we can cite the reference in this datasheet

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    Phoned looking for a control lysate for BCL9 antibody.

    Thank you for contacting us.

    We do not know what effect H2O2 stress will have on expression of BCL9 in MCF7 cells therefore I would recommend perhaps running H2O2 treated MCF7 lysate alongside untreated if possible to determine affect.
    ...

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    what exactly does MCF-7 stand for?

    Thank you for your enquiry. MCF stands for Michigan Cancer Foundation, the Detroit institution that derived this cell line. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Was this lysate diluted to achieve the final concentration of 2.5mg/ml? How much was it diluted? Does this extract contain DNA?

    Thank you for your enquiry. The MCF-7 nuclear extracts have been collected in Lysis Buffer. The Lysis Buffer consists of 20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM ß-glycerophosph...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"