Anti-MCHR antibody (ab21829)

Overview

• Product nameAnti-MCHR antibodySee all MCHR primary antibodies ...
• Description
  Mouse polyclonal to MCHR
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse
  
• Immunogen

  Fusion protein: NISDGQDNFTLAGPPPRTRS, corresponding to amino acids 16/35 of Mouse MCHR.

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• General notesProduced from outbred CD1 mice

  
  

  This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferConstituents: 50% Glycerol
• PurityWhole antiserum
• Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Neuroscience
  • Neurology process
  • Metabolism

Applications

Target

• RelevanceMCHR, a member of the G protein coupled receptor family 1, is an integral plasma membrane protein which binds melanin concentrating hormone. MCHR can inhibit cAMP accumulation and stimulate intracellular calcium flux, and is probably involved in the neuronal regulation of food consumption. Although structurally similar to somatostatin receptors, MCHR does not seem to bind somatostatin.
• Cellular localizationCell Membrane; multi-pass membrane protein
• Database links
  • Entrez Gene: 207911 Mouse
  • SwissProt: Q8JZL2 Mouse
  • Unigene: 323523 Mouse

• Alternative names
  G protein coupled receptor 24 antibodyG-protein coupled receptor 24 antibodyGPCR MCH1 antibody

  GPCR SLC/MCH1 antibodyGPR24 antibodyMCH 1R antibodyMCH receptor 1 antibodyMCH-1R antibodyMCH-R1 antibodyMCH1R antibodyMCHR antibodyMCHR-1 antibodyMelanin concentrating hormone receptor 1 antibodyMelanin-concentrating hormone receptor 1 antibodySLC-1 antibodySLC1 antibodySomatostatin receptor like protein antibody

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Anti-MCHR antibody images

• 

  Western blot - MCHR antibody (ab21829)
  All lanes : Anti-MCHR antibody (ab21829)
  
  Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen
  Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein
  
  Secondary
  Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
  
  Predicted band size : 46 kDa