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- Secondary antibodies
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Product name
Anti-MCK10 antibody
See all MCK10 products (6) ...
Description
Rabbit polyclonal to MCK10
Tested applications
IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 31-47 of Human MCK10.
Positive control
Placenta lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Primary antibody notes
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiogenesis >> Cardiomyocyte precursors
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Neuroscience >> Neurotransmission >> Receptors / Channels >> More Ion Channels
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
WB: 1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 105 kDa).
Target
Function
May be involved in cell-cell interactions and recognition.
Tissue specificity
Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 1 F5/8 type C domain.
Contains 1 protein kinase domain.
Domain
The Gly/Pro-rich domains may be required for an unusual geometry of interaction with ligand or substrates.
Cellular localization
Secreted and Membrane.
Target information above from: UniProt accessionQ08345
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CAK antibody
- CAK antibody
- CD 167 antibody
see all
Database links
- Entrez Gene: 780 Human
- Omim: 600408 Human
- SwissProt: Q08345 Human
- Unigene: 631988 Human
Anti-MCK10 antibody images:
Western blot - MCK10 antibody (ab5508)
Predicted band size : 105 kDa
ab5508 at a 1/100 dilution staining approximately 100kDa band of MCK 10 in placenta lysate by Western blot (ECL).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCK10 antibody (ab5508)
ab5508 staining MCK10 in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
References for Anti-MCK10 antibody (ab5508)
ab5508 has not yet been referenced specifically in any publications.
Publishing research using ab5508? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"