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Overview

  • Product nameMCK10 protein (Active)See all MCK10 proteins and peptides ...
  • Protein descriptionRecombinant fragment corresponding to amino acids 444-876 (cytoplasmic domain) of Human MCK10 with an N-terminal proprietary tag, expressed by Baculovirus in Sf9 insect cells; MWt 77 kDa (UniProt Q08345-2).
  • Uniprot accessionQ08345-2
  • Molecular weight77kDa inclusive of tags
  • Protein length433 amino acids
  • Expression hostBaculovirus

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Purification notesThe purity was determined to be >90% by densitometry
  • Biological activityThe specific activity of MCK10 is determined to be 5 nmol/min/mg
  • E.C. Number2.7.10.1
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab101591 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        CAKCD 167CD167
        CD167 antigen-like family member ACD167aCell adhesion kinaseDDRDDR 1DDR1DDR1_HUMANDiscoidin domain receptorDiscoidin domain receptor tyrosine kinase 1Discoidin receptor tyrosine kinaseDiscoidin receptor tyrosine kinase isoform aEDDR 1EDDR1Epithelial discoidin domain receptor 1Epithelial discoidin domain-containing receptor 1Epithelial specific receptor kinaseHGK2Mammarian carcinoma kinase 10Mammary carcinoma kinase 10MCK-10MCK10NEPNeuroepithelial tyrosine kinaseNeurotrophic tyrosine kinase receptor type 4NTRK 4NTRK4OTTHUMP00000029343OTTHUMP00000029344OTTHUMP00000029345OTTHUMP00000029346OTTHUMP00000029347OTTHUMP00000164863OTTHUMP00000164867OTTHUMP00000222080Protein-tyrosine kinase 3AProtein-tyrosine kinase RTK-6PTK 3PTK 3A protein tyrosine kinase 3APTK3PTK3AReceptor tyrosine kinase NEPRTK 6RTK6TRK ETRKETyrosine kinase DDRTyrosine kinase receptor ETyrosine-protein kinase CAK
      see all
  • FunctionMay be involved in cell-cell interactions and recognition.
  • Tissue specificityDetected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 1 F5/8 type C domain.
    Contains 1 protein kinase domain.
  • DomainThe Gly/Pro-rich domains may be required for an unusual geometry of interaction with ligand or substrates.
  • Cellular localizationSecreted and Membrane.

    • Target information above from: UniProt accession Q08345 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    MCK10 protein (Active) images

    • SDS-PAGE - MCK10 protein (ab101591)
      SDS-PAGE - MCK10 protein (ab101591)
      SDS-PAGE analysis of ab101591
    • Functional Studies - MCK10 protein (ab101591)
      Functional Studies - MCK10 protein (ab101591)
      Specific activity of ab101591

    References for MCK10 protein (Active) (ab101591)

    ab101591 has not yet been referenced specifically in any publications.

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