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Anti-MCL1 antibody [13] (ab5433)

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Overview

Product name

Anti-MCL1 antibody [13]
See all MCL1 products (21) ...

Description

Mouse monoclonal [13] to MCL1

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

A 327 amino acid, His-tagged C-terminal fusion protein taken from the rat protein sequence.

General notes

It is possible that a fine precipitant may form in this product.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: Tissue Culture Supernatant

Purity

Tissue culture supernatant

Primary antibody notes

Myeloid Cell Leukemia 1 (Mcl 1) is a member of a family of anti-apoptotic proteins related to Bcl-2 that include Bcl-2, Bcl-xL, Bcl-w, Bfl-1, BHRF-1 and CED-9. The Bcl-2 related family of proteins regulate apoptosis through the formation of both homo and heterodimers. Mcl 1 has been demonstrated to preferentially dimerize with the pro-apoptotic Bcl-2 family members which include Bax, Bak, Bok, Bik, and BOD. Rat and human Mcl 1 contain three Bcl-2 homology domains, denoted as BH1, BH2 and BH3, and is localized primarily to the mitochondria.

Clonality

Monoclonal

Clone number

13

Isotype

IgG1

Research areas

Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family

Applications

Show applications key

Our Abpromise guarantee covers the use of ab5433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

Application notes

WB: 1/10. Predicted molecular weight: detects a doublet at 40 kDa and 42 kDa representing Mcl 1 from human ML-1 cells.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

Sequence similarities

Belongs to the Bcl-2 family.

Post-translational
modifications

Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.

Cellular localization

Membrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.

Target information above from: UniProt accessionQ07820 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Bcl 2 related protein EAT/mcl1 antibody
  • Bcl-2-like protein 3 antibody
  • Bcl-2-related protein EAT/mcl1 antibody
  • BCL2 related antibody
  • Bcl2-L-3 antibody
  • BCL2L3 antibody
  • EAT antibody
  • Induced myeloid leukemia cell differentiation protein Mcl 1 antibody
  • Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
  • MCL 1 antibody
  • MCL1 antibody
  • MCL1-ES antibody
  • Mcl1/EAT antibody
  • MCL1_HUMAN antibody
  • MCL1L antibody
  • MCL1S antibody
  • MGC104264 antibody
  • MGC1839 antibody
  • Myeloid Cell Leukemia 1 antibody
  • myeloid cell leukemia sequence 1 antibody
  • Myeloid cell leukemia sequence 1 BCL2 related antibody
  • Myeloid cell leukemia sequence 1 isoform 1 antibody
  • OTTHUMP00000032794 antibody
  • OTTHUMP00000032795 antibody
  • TM antibody
see all

Database links

References for Anti-MCL1 antibody [13] (ab5433)

ab5433 has not yet been referenced specifically in any publications.

Publishing research using ab5433? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"