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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab101024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

Target

  • FunctionInvolved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • Post-translational
    modifications    Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
    Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
    Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
  • Cellular localizationMembrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.

    • Target information above from: UniProt accession Q07820 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Bcl 2 related protein EAT/mcl1 antibodyBcl-2-like protein 3 antibodyBcl-2-related protein EAT/mcl1 antibody
        BCL2 related antibodyBcl2-L-3 antibodyBCL2L3 antibodyEAT antibodyInduced myeloid leukemia cell differentiation protein Mcl 1 antibodyInduced myeloid leukemia cell differentiation protein Mcl-1 antibodyMCL 1 antibodyMCL1 antibodyMCL1-ES antibodyMcl1/EAT antibodyMCL1_HUMAN antibodyMCL1L antibodyMCL1S antibodyMGC104264 antibodyMGC1839 antibodyMyeloid Cell Leukemia 1 antibodyMyeloid cell leukemia ES antibodyMyeloid cell leukemia sequence 1 antibodyMyeloid cell leukemia sequence 1 BCL2 related antibodyMyeloid cell leukemia sequence 1 isoform 1 antibodyOTTHUMP00000032794 antibodyOTTHUMP00000032795 antibodyTM antibody
      see all

    Anti-MCL1 antibody images

    • Western blot - MCL1 antibody (ab101024)
      Western blot - MCL1 antibody (ab101024)
      All lanes : Anti-MCL1 antibody (ab101024) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 37 kDa
      Observed band size : 37 kDa
      Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 20 minutes

    References for Anti-MCL1 antibody (ab101024)

    ab101024 has not yet been referenced specifically in any publications.

    Publishing research using ab101024 ? Please let us know so that we can cite the reference in this datasheet

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