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- Secondary antibodies
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Product name
Anti-MCL1 antibody
See all MCL1 products (21) ...
Description
Rabbit polyclonal to MCL1
Specificity
This antibody detects an ~42/43 kDa protein doublet, corresponding to the expected molecular mass of Mcl1 on SDS-PAGE immunoblots. The antibody specificity is confirmed by peptide inhibition studies. An additional protein at ~33kDa may also be detected. Other additional proteins may also be seen in some samples.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Rabbit, Guinea pig, Dog, Human, Pig, Monkey
Immunogen
Synthetic peptide: SPEEELDGYEPEPLGKRPA conjugated to KLH, corresponding to amino acids 121-139 of Human MCL1
speeeldgyepeplgkrpa
Positive control
HeLa heat shocked cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 8.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at an assay dependent dilution.
WB: Use at a concentration of 0.1 µg/ml. This concentration was sufficient for detection of Mcl1 in 20µg of HeLa Heat Shocked Cell Lysate by ECL immunoblot analysis. Predicted molecular weight: 42-43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
Sequence similarities
Belongs to the Bcl-2 family.
Post-translational
modifications
Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
Cellular localization
Membrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
Target information above from: UniProt accessionQ07820
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Bcl 2 related protein EAT/mcl1 antibody
- Bcl-2-like protein 3 antibody
- Bcl-2-related protein EAT/mcl1 antibody
see all
Database links
- Entrez Gene: 4170 Human
- Omim: 159552 Human
- SwissProt: Q07820 Human
- Unigene: 632486 Human
Anti-MCL1 antibody images:
Immunocytochemistry/ Immunofluorescence - MCL1 antibody (ab28147)
ab28147 staining MCL1 in human Hella cells by Immunocytochemistry/ immunofluorescence.
- MCL1 antibody (ab28147)
ab28147 at 1/50 dillution staining MCL1 in human breast cancer tissue section by Immunohistochemistry.
References for Anti-MCL1 antibody (ab28147)
ab28147 has not yet been referenced specifically in any publications.
Publishing research using ab28147? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"