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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-MCM10 antibody
See all MCM10 products (2) ...
Description
Rabbit polyclonal to MCM10
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Does not react with
Xenopus laevis, Fruit fly (Drosophila melanogaster)
Immunogen
Synthetic peptide (Human) - which represented a portion of Minichromosome Maintenance Deficient 10 encoded within exon 14.
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Western blot - MCM10 antibody (ab3733)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use a concentration of 1 - 4 µg/ml.
WB: 1/1000 - 1/5000.Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
Target
Function
Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication.
Sequence similarities
Belongs to the MCM10 family.
Developmental stage
Expression is cell cycle regulated. Expression increases at the G1/S-boundary. Expression decreases in late M phase, remains low during G(1) phase, and starts to accumulate at the onset of S phase.
Domain
The zinc finger-like domain binds a zinc ion and is involved in self-association.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus. Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli.
Target information above from: UniProt accessionQ7L590
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ31133 antibody
- HsMCM10 antibody
- mcm10 antibody
see all
Database links
- Entrez Gene: 55388 Human
- Entrez Gene: 70024 Mouse
- Omim: 609357 Human
- SwissProt: Q3MIR3 Human
- SwissProt: Q5T670 Human
- SwissProt: Q7L590 Human
- SwissProt: Q9H0D7 Human
- SwissProt: Q9H3P9 Human
see all
Anti-MCM10 antibody images:
Western blot - MCM10 antibody (ab3733)
Predicted band size : 110 kDa
Sample: Hela cell lysate (1mg for IP).
Antibody: Affinity purified Rabbit anti-MCM 10 (ab3733); 1mg for IP, 1 / 2000 dilution for WB.
Detection: ECL.
References for Anti-MCM10 antibody (ab3733)
This product has been referenced in:
- Im JSet al. Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins. Proc Natl Acad Sci U S A 106:15628-32 (2009).Read more (PubMed: 19805216) »
See 1 publication for this product
Publishing research using ab3733? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"