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Western blot - MCM10 antibody (ab3733)
(enlarge)
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Product Name
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MCM10 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MCM10
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Immunogen
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Synthetic peptide (Human) - which represented a portion of Minichromosome Maintenance Deficient 10 encoded within exon 14.
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Reacts with
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Hu
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Does not react with
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Dm, Xl
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at a concentration of 1 - 4 µg/ml.
WB: 1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Relevance
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MCM10 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MCM10 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MCM10 antibody images, references, products related to ab3733 and other tools.
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MCM10 antibody images:
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 Western blot - MCM10 antibody (ab3733)
Predicted band size : 110 kDa
Sample: Hela cell lysate (1mg for IP). Antibody: Affinity purified Rabbit anti-MCM 10 (ab3733); 1mg for IP, 1 / 2000 dilution for WB. Detection: ECL.
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References for MCM10 antibody (ab3733)
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This product has been used in:
Im JS et al. Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins. Proc Natl Acad Sci U S A 106:15628-32 (2009). PubMed: 19805216
If you publish research using ab3733 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MCM10
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MCM10 antibody - more information
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Customer reviews (feedback) regarding MCM10 antibody |
Customer FAQs regarding MCM10 antibody |
Protocols for MCM10 antibody |
Price and availability of products related to MCM10 antibody |
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MCM10 antibody - related products:
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MCM10 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MCM10 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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