Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ] (ab6153)

Overview

• Product nameAnti-MCM2 antibody [CRCT2.1 (D1.9H5) ]See all MCM2 primary antibodies ...
• Description
  Mouse monoclonal [CRCT2.1 (D1.9H5) ] to MCM2
• SpecificityRecognises human Mcm2
• Tested applicationsIHC-P, IHC-Fr, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Bacterially expressed human Mcm2

• Positive controlTonsil, any tissue with high proliferative index
• General notesClone Number: CRCT2.1 (D1.9H5)

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS with 0.1% sodium azide
• Concentration information loading...
• Purification notesPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
• Clonality Monoclonal
• Clone numberCRCT2.1 (D1.9H5)
• MyelomaSp2
• IsotypeIgG1
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Synthesis
  • Other

Applications

Target

• FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
• Sequence similaritiesBelongs to the MCM family.
  Contains 1 MCM domain.
• Post-translational
  modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
• Cellular localizationNucleus.

Target information above from: UniProt accession P49736 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4171 Human
  • Omim: 116945 Human
  • SwissProt: P49736 Human
  • Unigene: 477481 Human

• Alternative names
  BM28 antibodyCCNL 1 antibodyCCNL1 antibody

  CDC like 1 antibodyCDC like-1 antibodycdc19 antibodyCDCL 1 antibodyCDCL1 antibodyCell devision cycle like 1 antibodyCyclin like 1 antibodycyclin like-1 antibodyD3S3194 antibodyDNA replication licensing factor MCM2 antibodyKIAA0030 antibodyMCM 2 antibodyMCM2 antibodyMCM2 minichromosome maintenance deficient 2 mitotin antibodyMCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibodyMCM2 minichromosome maintenance deficient 2, mitotin antibodyMCM2_HUMAN antibodyMGC10606 antibodyMinichromosome maintenance complex component 2 antibodyMinichromosome maintenance deficient 2 (mitotin) antibodyMinichromosome maintenance deficient 2 mitotin antibodyMinichromosome maintenance protein 2 antibodyMinichromosome maintenance protein 2 homolog antibodyMitotin antibodyNuclear protein BM28 antibodyOTTHUMP00000216047 antibodyOTTHUMP00000216050 antibody

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Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ] images

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  Western blot - MCM2 antibody [CRCT2.1 (D1.9H5) ] (ab6153)
  
  
  Predicted band size : 100 kDa
  
  
  Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.