MCM2 antibody [CRCT2.1 (D1.9H5) ] (ab6153)

Product Name 

MCM2 antibody [CRCT2.1 (D1.9H5) ]

Product type 

Primary antibodies

Description 

Mouse monoclonal [CRCT2.1 (D1.9H5) ] to MCM2

Immunogen 

Bacterially expressed human Mcm2

Reacts with 
(species key)

Hu

Specificity 

Recognises human Mcm2

Tested applications 
(see key)

IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/500 - 1/1000.
WB: Use at an assay dependent dilution. Detects a band of approximately ~110 kDa (predicted molecular weight: 100 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Tonsil, any tissue with high proliferative index

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other

Relevance 

MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. It is similar to members of the family of early S phase proteins. Mincheva et al. (1994) mapped the gene to 3q21. From its localization, CDCL1 became a candidate for an oncogene affected by chromosomal breaks in acute myeloid leukemia (AML)

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

CRCT2.1 (D1.9H5)

Myeloma 

Sp2

Isotype 

IgG1

Storage buffer 

PBS with 0.1% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Clone Number: CRCT2.1 (D1.9H5)

See below for MCM2 antibody [CRCT2.1 (D1.9H5) ] images, references, products related to ab6153 and other tools.

MCM2 antibody [CRCT2.1 (D1.9H5) ] images:

References for MCM2 antibody [CRCT2.1 (D1.9H5) ] (ab6153)

  This product has been used in: (two most recent references)

Sarfraz S et al. Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection. BMC Microbiol 8:133 (2008). IHC-P; Human. PubMed: 18680610

Villadsen R et al. Evidence for a stem cell hierarchy in the adult human breast. J Cell Biol 177:87-101 (2007). PubMed: 17420292

See all 4 publication references for this product.


If you publish research using ab6153 please let us know so that we can cite the reference on this datasheet.

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