Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ] (ab6153)
- Product nameAnti-MCM2 antibody [CRCT2.1 (D1.9H5) ]See all MCM2 primary antibodies ...
- DescriptionMouse monoclonal [CRCT2.1 (D1.9H5) ] to MCM2
- SpecificityRecognises human Mcm2
- Tested applicationsIHC-P, IHC-Fr, WB more details
- Species reactivityReacts with: Human
Bacterially expressed human Mcm2
- Positive control
- Tonsil, any tissue with high proliferative index
- General notesClone Number: CRCT2.1 (D1.9H5)
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPBS with 0.1% sodium azide
- Concentration information loading...
- Purification notesPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Clonality Monoclonal
- Clone numberCRCT2.1 (D1.9H5)
- Research Areas
Our Abpromise guarantee covers the use of ab6153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||IHC-Fr: 1/500 - 1/1000.|
|WB||WB: Use at an assay dependent dilution. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa).|
- FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
- Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain.
modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
- Cellular localizationNucleus.
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Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ] images
Predicted band size : 100 kDa
Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.
References for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ] (ab6153)
This product has been referenced in:
- Shtutman M et al. Function-based gene identification using enzymatically generated normalized shRNA library and massive parallel sequencing. Proc Natl Acad Sci U S A 107:7377-82 (2010). WB ; Human . Read more (PubMed: 20368428) »
- Sarfraz S et al. Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection. BMC Microbiol 8:133 (2008). IHC-P ; Human . Read more (PubMed: 18680610) »