Overview

  • Product nameAnti-MCM2 antibody [EPR4120]
    See all MCM2 primary antibodies
  • Description
    Rabbit monoclonal [EPR4120] to MCM2
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MCM2.

  • Positive control
    • WB: MCF-7, Ramos, SW480, Molt-4, Jurkat and HeLa cell lysates IHC-P: Human squamous cell cervical carcinoma tissue and Human tonsil tissue IF: HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD) KD = 5.80 x 10 -11 M Learn more about KD
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Monoclonal
  • Clone numberEPR4120
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab108935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 102 kDa.

For unpurified, use 1/1000 - 1/5000.

IHC-P 1/200.

For unpurified, use 1/100.

ICC/IF 1/600.

For unpurified, use 1/250.

Target

  • FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
  • Sequence similaritiesBelongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BM28 antibody
    • CCNL 1 antibody
    • CCNL1 antibody
    • CDC like 1 antibody
    • cdc19 antibody
    • CDCL 1 antibody
    • CDCL1 antibody
    • Cell devision cycle like 1 antibody
    • Cyclin like 1 antibody
    • D3S3194 antibody
    • DNA replication licensing factor MCM2 antibody
    • KIAA0030 antibody
    • MCM 2 antibody
    • MCM2 antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
    • MCM2 minichromosome maintenance deficient 2, mitotin antibody
    • MCM2_HUMAN antibody
    • MGC10606 antibody
    • Minichromosome maintenance complex component 2 antibody
    • Minichromosome maintenance deficient 2 (mitotin) antibody
    • Minichromosome maintenance deficient 2 mitotin antibody
    • Minichromosome maintenance protein 2 antibody
    • Minichromosome maintenance protein 2 homolog antibody
    • Mitotin antibody
    • Nuclear protein BM28 antibody
    • OTTHUMP00000216047 antibody
    • OTTHUMP00000216050 antibody
    see all

Anti-MCM2 antibody [EPR4120] images

  • All lanes : Anti-MCM2 antibody [EPR4120] (ab108935) at 1/8500 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Mouse kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 102 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-MCM2 antibody [EPR4120] (ab108935) at 1/8500 dilution (purified) + Jurkat cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 102 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human tonsil with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

  • Immunohistochemical staining of paraffin embedded rat liver with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

  • Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab108935 at a dilution of 1/600. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

  • All lanes : Anti-MCM2 antibody [EPR4120] (ab108935) at 1/5000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Mouse kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP goat anti-rabbit (H+L) at 1000

    Predicted band size : 102 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-MCM2 antibody [EPR4120] (ab108935) at 1/10000 dilution (unpurified)

    Lane 1 : MCF-7 cell lysate
    Lane 2 : Ramos cell lysate
    Lane 3 : SW480 cell lysate
    Lane 4 : Molt-4 cell lysate
    Lane 5 : Jurkat cell lysate
    Lane 6 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 102 kDa
  • Immunohistochemical staining of MCM2 in paraffin-embedded human squamous cell cervical carcinoma tissue with unpurified ab108395, at a 1/250 dilution.

  • Immunohistochemical staining of MCM2 in paraffin-embedded human tonsil tissue with unpurified ab108395, at a 1/250 dilution.

  • Immunohistochemical staining of paraffin embedded rat liver with unpurified ab108935 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

  • Unpurified ab108935, at a 1/100 dilution, staining MCM2 in HeLa cells.

  • Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab108935 at a dilution of 1/250. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-MCM2 antibody [EPR4120] (ab108935)

ab108935 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"