Anti-MCM2 antibody [EPR4120] (ab108935)

Overview

• Product nameAnti-MCM2 antibody [EPR4120]See all MCM2 primary antibodies ...
• Description
  Rabbit monoclonal [EPR4120] to MCM2
• Tested applicationsWB, IP, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human
  

  Does not react with

  Rat
• Immunogen

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

• Positive controlWB: MCF-7, Ramos, SW480, Molt-4, Jurkat and HeLa cell lysates IHC-P: Human squamous cell cervical carcinoma tissue and Human tonsil tissue IF: HeLa cells
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Dissociation constant (Kd) Kd = 5.80 x 10^-11 M
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR4120
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Synthesis
  • Other

Applications

Target

• FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
• Sequence similaritiesBelongs to the MCM family.
  Contains 1 MCM domain.
• Post-translational
  modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
• Cellular localizationNucleus.

Target information above from: UniProt accession P49736 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4171 Human
  • Entrez Gene: 17216 Mouse
  • Omim: 116945 Human
  • SwissProt: P49736 Human
  • SwissProt: P97310 Mouse
  • Unigene: 477481 Human
  • Unigene: 16711 Mouse

• Alternative names
  BM28 antibodyCCNL 1 antibodyCCNL1 antibody

  CDC like 1 antibodycdc19 antibodyCDCL 1 antibodyCDCL1 antibodyCell devision cycle like 1 antibodyCyclin like 1 antibodyD3S3194 antibodyDNA replication licensing factor MCM2 antibodyKIAA0030 antibodyMCM 2 antibodyMCM2 antibodyMCM2 minichromosome maintenance deficient 2 mitotin antibodyMCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibodyMCM2 minichromosome maintenance deficient 2, mitotin antibodyMCM2_HUMAN antibodyMGC10606 antibodyMinichromosome maintenance complex component 2 antibodyMinichromosome maintenance deficient 2 (mitotin) antibodyMinichromosome maintenance deficient 2 mitotin antibodyMinichromosome maintenance protein 2 antibodyMinichromosome maintenance protein 2 homolog antibodyMitotin antibodyNuclear protein BM28 antibodyOTTHUMP00000216047 antibodyOTTHUMP00000216050 antibody

  see all

Anti-MCM2 antibody [EPR4120] images

• 

  Western blot - MCM2 antibody [EPR4120] (ab108935)
  All lanes : Anti-MCM2 antibody [EPR4120] (ab108935) at 1/10000 dilution
  
  Lane 1 : MCF-7 cell lysate
  Lane 2 : Ramos cell lysate
  Lane 3 : SW480 cell lysate
  Lane 4 : Molt-4 cell lysate
  Lane 5 : Jurkat cell lysate
  Lane 6 : HeLa cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 102 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 antibody [EPR4120] (ab108935)
  ab108395, at a 1/250 dilution, staining MCM2 in paraffin-embedded Human squamous cell cervical carcinoma tissue
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 antibody [EPR4120] (ab108935)
  ab108395, at a 1/250 dilution, staining MCM2 in paraffin-embedded Human tonsil tissue
• 

  Immunocytochemistry/ Immunofluorescence - MCM2 antibody [EPR4120] (ab108935)
  ab108935, at a 1/100 dilution, staining MCM2 in HeLa cells
• 

  Other-Anti-MCM2 antibody [EPR4120](ab108935)
  Equilibrium disassociation constant (K_D)
  Learn more about K_D
  
  Click here to learn more about K_D