Overview

  • Product name
    Anti-MCM2 antibody [SP85]
    See all MCM2 primary antibodies
  • Description
    Rabbit monoclonal [SP85] to MCM2
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide within Human MCM2 aa 120-260 (internal sequence). The exact sequence is proprietary.
    Database link: P49736

  • Positive control
    • Human rectal carcinoma tissue, HeLa cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, 0.0268% PBS
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Protein A/G purified
  • Clonality
    Monoclonal
  • Clone number
    SP85
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P 1/100. Antigen retrieval is recommended by the following method: boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min.
Flow Cyt Use at an assay dependent concentration. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
  • Sequence similarities
    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BM28 antibody
    • CCNL 1 antibody
    • CCNL1 antibody
    • CDC like 1 antibody
    • cdc19 antibody
    • CDCL 1 antibody
    • CDCL1 antibody
    • Cell devision cycle like 1 antibody
    • Cyclin like 1 antibody
    • D3S3194 antibody
    • DNA replication licensing factor MCM2 antibody
    • KIAA0030 antibody
    • MCM 2 antibody
    • MCM2 antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
    • MCM2 minichromosome maintenance deficient 2, mitotin antibody
    • MCM2_HUMAN antibody
    • MGC10606 antibody
    • Minichromosome maintenance complex component 2 antibody
    • Minichromosome maintenance deficient 2 (mitotin) antibody
    • Minichromosome maintenance deficient 2 mitotin antibody
    • Minichromosome maintenance protein 2 antibody
    • Minichromosome maintenance protein 2 homolog antibody
    • Mitotin antibody
    • Nuclear protein BM28 antibody
    • OTTHUMP00000216047 antibody
    • OTTHUMP00000216050 antibody
    see all

Images

  • Anti-MCM2 antibody [SP85] (ab95361) at 1/100 dilution + lysate prepared from HeLa cells

    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
  • ab95361, at 1/100 dilution, staining MCM2 in formalin-fixed, paraffin-embedded Human rectal carcinoma tissue by Immunohistochemistry.
  • Flow cytometric analysis using rabbit anti-MCM2 (SP85) antibody ab95361(1/100) in HeLa cells (green) compared to negative control of rabbit IgG (blue).

  • ab95361,at 1/100 dilution,staining MCM2 in formalin-fixed,paraffin-embedded Human pancreas adenocarcinoma tissue by Immunohistochemistry.

  • ab95361,at 1/100 dilution,staining MCM2 in formalin-fixed,paraffin-embedded Human prostate tissue by Immunohistochemistry.

References

ab95361 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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