Anti-MCM2 antibody [SPM196], prediluted (ab17788)

Overview

• Product nameAnti-MCM2 antibody [SPM196], predilutedSee all MCM2 primary antibodies ...
• Description
  Mouse monoclonal [SPM196] to MCM2, prediluted
• Specificityab17788 detects a 120-125kDa protein corresponding to human MCM2
• Tested applicationsIHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Bacterially expressed human MCM2

• Positive controlHela cells. Tonsil
• General notes

  
  

  MCM proteins have DNA dependent ATPase motifs in their central domain which is conserved from yeast to mammals. MCM proteins form a complex which exhibits ATPase and helicase activities. In G0 phase levels of MCM 2 and 5 are lower than that of MCM7 and 3 but increases as the cell enters G1/S phase of the cell cycle.

Properties

• FormPrediluted
• Storage instructionsStore at +4°C.
• Concentration information loading...
• PurityIgG fraction
• Primary antibody notes MCM proteins have DNA dependent ATPase motifs in their central domain which is conserved from yeast to mammals. MCM proteins form a complex which exhibits ATPase and helicase activities. In G0 phase levels of MCM 2 and 5 are lower than that of MCM7 and 3 but increases as the cell enters G1/S phase of the cell cycle.
• Clonality Monoclonal
• Clone numberSPM196
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Synthesis
  • Other

Applications

Target

• FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
• Sequence similaritiesBelongs to the MCM family.
  Contains 1 MCM domain.
• Post-translational
  modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
• Cellular localizationNucleus.

Target information above from: UniProt accession P49736 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4171 Human
  • Omim: 116945 Human
  • SwissProt: P49736 Human
  • Unigene: 477481 Human

• Alternative names
  BM28 antibodyBM28 antibodyCCNL 1 antibody

  CCNL1 antibodyCCNL1 antibodyCDC like 1 antibodyCDC like-1 antibodycdc19 antibodycdc19 antibodyCDCL 1 antibodyCDCL1 antibodyCDCL1 antibodyCell devision cycle like 1 antibodyCyclin like 1 antibodycyclin like-1 antibodycyclin like-1 antibodyD3S3194 antibodyD3S3194 antibodyDNA replication licensing factor MCM2 antibodyKIAA0030 antibodyKIAA0030 antibodyMCM 2 antibodyMCM2 antibodyMCM2 minichromosome maintenance deficient 2 mitotin antibodyMCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibodyMCM2 minichromosome maintenance deficient 2, mitotin antibodyMCM2_HUMAN antibodyMGC10606 antibodyMinichromosome maintenance complex component 2 antibodyMinichromosome maintenance deficient 2 (mitotin) antibodyMinichromosome maintenance deficient 2 mitotin antibodyMinichromosome maintenance protein 2 antibodyMinichromosome maintenance protein 2 homolog antibodyMitotin antibodyMitotin antibodyNuclear protein BM28 antibodyNuclear protein BM28 antibodyOTTHUMP00000216047 antibodyOTTHUMP00000216050 antibody

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Anti-MCM2 antibody [SPM196], prediluted images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 antibody [SPM196], prediluted (ab17788)
  ab17788 staining MCM2 in Human tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). ab17788 was incubated for 30 minutes at room temperature.
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 antibody [SPM196], prediluted (ab17788)This image is courtesy of an Abreview submitted by Mrs Melissa Downing
  ab17788 used undiluted staining human tonsil tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed in citrate buffer before incubation with the antibody for 1 hour. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

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