Anti-MCM2 antibody [SPM196], prediluted (ab17788)

Overview

  • Product nameAnti-MCM2 antibody [SPM196], predilutedSee all MCM2 primary antibodies ...
  • Description
    Mouse monoclonal [SPM196] to MCM2, prediluted
  • Specificityab17788 detects a 120-125kDa protein corresponding to human MCM2
  • Tested applicationsIHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Bacterially expressed human MCM2

  • Positive control
    • Hela cells. Tonsil
  • General notes


    MCM proteins have DNA dependent ATPase motifs in their central domain which is conserved from yeast to mammals. MCM proteins form a complex which exhibits ATPase and helicase activities. In G0 phase levels of MCM 2 and 5 are lower than that of MCM7 and 3 but increases as the cell enters G1/S phase of the cell cycle.

Properties

  • FormPrediluted
  • Storage instructionsStore at +4°C.
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notes MCM proteins have DNA dependent ATPase motifs in their central domain which is conserved from yeast to mammals. MCM proteins form a complex which exhibits ATPase and helicase activities. In G0 phase levels of MCM 2 and 5 are lower than that of MCM7 and 3 but increases as the cell enters G1/S phase of the cell cycle.
  • Clonality Monoclonal
  • Clone numberSPM196
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab17788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
  • Sequence similaritiesBelongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession P49736 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • BM28 antibody
    • BM28 antibody
    • CCNL 1 antibody
    • CCNL1 antibody
    • CCNL1 antibody
    • CDC like 1 antibody
    • CDC like-1 antibody
    • cdc19 antibody
    • cdc19 antibody
    • CDCL 1 antibody
    • CDCL1 antibody
    • CDCL1 antibody
    • Cell devision cycle like 1 antibody
    • Cyclin like 1 antibody
    • cyclin like-1 antibody
    • cyclin like-1 antibody
    • D3S3194 antibody
    • D3S3194 antibody
    • DNA replication licensing factor MCM2 antibody
    • KIAA0030 antibody
    • KIAA0030 antibody
    • MCM 2 antibody
    • MCM2 antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
    • MCM2 minichromosome maintenance deficient 2, mitotin antibody
    • MCM2_HUMAN antibody
    • MGC10606 antibody
    • Minichromosome maintenance complex component 2 antibody
    • Minichromosome maintenance deficient 2 (mitotin) antibody
    • Minichromosome maintenance deficient 2 mitotin antibody
    • Minichromosome maintenance protein 2 antibody
    • Minichromosome maintenance protein 2 homolog antibody
    • Mitotin antibody
    • Mitotin antibody
    • Nuclear protein BM28 antibody
    • Nuclear protein BM28 antibody
    • OTTHUMP00000216047 antibody
    • OTTHUMP00000216050 antibody
    see all

Anti-MCM2 antibody [SPM196], prediluted images

  • ab17788 staining MCM2 in Human tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). ab17788 was incubated for 30 minutes at room temperature.
  • ab17788 used undiluted staining human tonsil tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed in citrate buffer before incubation with the antibody for 1 hour. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

References for Anti-MCM2 antibody [SPM196], prediluted (ab17788)

ab17788 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tonsil)
Specification tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1.5%
Username

Mrs. Melissa Downing

Verified customer

Submitted Aug 14 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"