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ab31159 has been referenced in 7 publications.
Publishing research using ab31159? Please let us know so that we can cite the reference in this datasheet
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Immunohistochemical analysis of MCM2 expression in formalin fixed paraffin embedded human breast carcinoma tissue section, using 1/100 ab31159.
Predicted band size : 102 kDa
Soluble or chromatin-bound protein prepared from MEFs was electrophoresed on PAGE gels, electrotransferred, and the blots were immunolabeled with ab31159. The bands correspond to the predicted molecular weights of these proteins, and for MCM2, the identity of the band was verifed by RNAi knockdown and transient overexpression in NIH 3T3 cells.
Image from Chuang CH et al, PLoS Genet. 2010 Sep 9;6(9). pii: e1001110, Fig 1.
ab31159 staining MCM2in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton 0.1% in PBS and blocked with 3% BSA for 1 hour at 24ºC. Samples were incubated with primary antibody (1/100 in PBS + 3% BSA) for 1 hour at 37ºC. An Alexa Fluor®555-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were counterstained in blue.
This image is courtesy of an Abreview submitted by Nina Marinsek
ab31159 staining MCM2 in human tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using EDTA pH9.0. Samples were then incubated with ab31159 at a 1/300 for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse-rabbit polyclonal.
This image is courtesy of an anonymous Abreview.
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