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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - MCM2 antibody (ab31159)
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Product Name
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MCM2 antibody
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See all MCM2 antibodies (17)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MCM2
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Immunogen
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Synthetic peptide corresponding to N terminal of human MCM2 protein.
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat
(Due to sequence homology)
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/100 for 30 min at RT. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB: Use at an assay dependent dilution. Predicted molecular weight: 102 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cells. Breast carcinoma.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Relevance
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MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. It is similar to members of the family of early S phase proteins. Mincheva et al. (1994) mapped the gene to 3q21. From its localization, CDCL1 became a candidate for an oncogene affected by chromosomal breaks in acute myeloid leukemia (AML)
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MCM2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MCM2 antibody images, references, products related to ab31159 and other tools.
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MCM2 antibody images:
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 Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - MCM2 antibody (ab31159)
Immunohistochemical analysis of MCM2 expression in formalin fixed paraffin embedded human breast carcinoma tissue section, using 1/100 ab31159.
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References for MCM2 antibody (ab31159)
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This product has been used in:
Graham JD et al. DNA replication licensing and progenitor numbers are increased by progesterone in normal human breast. Endocrinology 150:3318-26 (2009). IHC-P; Human. PubMed: 19342456
If you publish research using ab31159 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MCM2
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MCM2 antibody - more information
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Customer reviews (feedback) regarding MCM2 antibody |
Customer FAQs regarding MCM2 antibody |
Protocols for MCM2 antibody |
Price and availability of products related to MCM2 antibody |
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MCM2 antibody - related products:
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MCM2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MCM2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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