Overview

  • Product nameAnti-MCM2 antibodySee all MCM2 primary antibodies ...
  • Description
    Rabbit polyclonal to MCM2
  • Tested applicationsICC, ICC/IF, WB, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to N terminal of human MCM2 protein.

  • Positive control
    • HeLa cells. Breast carcinoma.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab31159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 102 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Target

  • FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
  • Sequence similaritiesBelongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession P49736 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • BM28 antibody
    • CCNL 1 antibody
    • CCNL1 antibody
    • CDC like 1 antibody
    • CDC like-1 antibody
    • cdc19 antibody
    • CDCL 1 antibody
    • CDCL1 antibody
    • Cell devision cycle like 1 antibody
    • Cyclin like 1 antibody
    • cyclin like-1 antibody
    • cyclin like-1 antibody
    • D3S3194 antibody
    • D3S3194 antibody
    • DNA replication licensing factor MCM2 antibody
    • KIAA0030 antibody
    • KIAA0030 antibody
    • MCM 2 antibody
    • MCM2 antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
    • MCM2 minichromosome maintenance deficient 2, mitotin antibody
    • MCM2_HUMAN antibody
    • MGC10606 antibody
    • Minichromosome maintenance complex component 2 antibody
    • Minichromosome maintenance deficient 2 (mitotin) antibody
    • Minichromosome maintenance deficient 2 mitotin antibody
    • Minichromosome maintenance protein 2 antibody
    • Minichromosome maintenance protein 2 homolog antibody
    • Mitotin antibody
    • Nuclear protein BM28 antibody
    • OTTHUMP00000216047 antibody
    • OTTHUMP00000216050 antibody
    see all

Anti-MCM2 antibody images

  • Immunohistochemical analysis of MCM2 expression in formalin fixed paraffin embedded human breast carcinoma tissue section, using 1/100 ab31159.


  • Predicted band size : 102 kDa

    Image from Chuang CH et al, PLoS Genet. 2010 Sep 9;6(9). pii: e1001110, Fig 1.

  • ab31159 staining MCM2in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton 0.1% in PBS and blocked with 3% BSA for 1 hour at 24°C. Samples were incubated with primary antibody (1/100 in PBS + 3% BSA) for 1 hour at 37°C. An Alexa Fluor®555-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were counterstained in blue.

    See Abreview

  • ab31159 staining MCM2 in human tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using EDTA pH9.0. Samples were then incubated with ab31159 at a 1/300 for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse-rabbit polyclonal.

    See Abreview

References for Anti-MCM2 antibody (ab31159)

This product has been referenced in:
  • Thiolloy S  et al. An osteoblast-derived proteinase controls tumor cell survival via TGF-beta activation in the bone microenvironment. PLoS One 7:e29862 (2012). IHC-P ; Human . Read more (PubMed: 22238668) »
  • He Z  et al. Optimization of immunolocalization of cell cycle proteins in human corneal endothelial cells. Mol Vis 17:3494-511 (2011). IHC - Wholemount ; Human . Read more (PubMed: 22219645) »

See all 9 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Human Cultured Cells (corneal endothelial cells in vitro)
Specification corneal endothelial cells in vitro
Fixative Formaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Dr. Zhiguo He

Verified customer

Submitted Nov 06 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (corneal epithelium)
Specification corneal epithelium
Fixative Paraformaldehyde
Permeabilization Yes - 1% Triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Dr. Zhiguo He

Verified customer

Submitted Nov 05 2012

The concentrations of those lots are as follows:

GR71584-3 is 0.12 mg/ml.
GR90115-1 is 0.17 mg/ml.
GR91461-1 is 0.16 mg/ml

I hope this helps. Please contact us if you have other questions.

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tonsil)
Specification tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 20 min, 100C
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Nov 03 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton/PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Miss. Nina Marinsek

Verified customer

Submitted Nov 23 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin tissue section from paraffin embedded sample)
Specification Skin tissue section from paraffin embedded sample
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5%
Username

Dr. Julie Richards

Verified customer

Submitted May 31 2007

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10%
Username

Dr. Ahmed Elsharkawy

Verified customer

Submitted Dec 12 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"