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Our end user want to obtain some reference (publish all valid customer reviews (valid means enough data and proof of purchase). Please send me some information about ab4461 or 4458.
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ANSWER: |
Thanks for your enquiry. Unfortunately, we are not aware of any publications that feature the use of either of these antibodies. We also have not received any feedback from customers in the form of reviews who have used these antibodies. For both ab4461 and ab4458, there is a nice Western image on the online datasheets. However, that is all the information that we have at this time. Please let me know if you have any additional questions. |
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I am going to buy your products, MCM2 antibody. But I found several same antibody from your company. This antibody will be used in Western blot. Could you tell me what difference between them(such as, ab1896, ab6153,ab4461)? Are they all antibody for phosphoMCM2? Could you help me to decide which I had better to choose? Many thanks
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ANSWER: |
All the information available for this product is listed on the on-line datasheet (price, datasheet, immunogen, publication, suitability, cross-reactivity). I suggest you take a look at the datasheets for these particular products in order to ascertain their level of usefulness in your experimental procedures. I'm sorry for not being able to give you more guidance. If you require additional information to that provided on the product datasheet, please contact us again and we will gladly assist you. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 100 kDa
Sample: HeLa cell RIPA extract.
WB - 50 µg.
CoIP - 7 µg.
ab4461 incubated at 0.2 µg/ml for 1 hour for WB.
ab4461 incubated at 20 µg for CoIP.
Detection:
WB -ECL
CoIP - Coomassie Stain
ICC/IF image of ab4461 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4461, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Ab4461 staining MCM2 in Human placenta.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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