Overview

  • Product nameAnti-MCM2 antibodySee all MCM2 primary antibodies ...
  • Description
    Rabbit polyclonal to MCM2
  • Tested applicationsIHC-Fr, ICC/IF, IHC-P, WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Human, Xenopus laevis
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (Human) - 37 amino acids which represent a portion of human MCM 2 encoded within exon 2.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab4461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 21068061
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 100 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC Use at an assay dependent concentration.

Target

  • FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
  • Sequence similaritiesBelongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • BM28 antibody
    • CCNL 1 antibody
    • CCNL1 antibody
    • CDC like 1 antibody
    • CDC like-1 antibody
    • cdc19 antibody
    • CDCL 1 antibody
    • CDCL1 antibody
    • Cell devision cycle like 1 antibody
    • Cyclin like 1 antibody
    • cyclin like-1 antibody
    • D3S3194 antibody
    • DNA replication licensing factor MCM2 antibody
    • KIAA0030 antibody
    • MCM 2 antibody
    • MCM2 antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
    • MCM2 minichromosome maintenance deficient 2, mitotin antibody
    • MCM2_HUMAN antibody
    • MGC10606 antibody
    • Minichromosome maintenance complex component 2 antibody
    • Minichromosome maintenance deficient 2 (mitotin) antibody
    • Minichromosome maintenance deficient 2 mitotin antibody
    • Minichromosome maintenance protein 2 antibody
    • Minichromosome maintenance protein 2 homolog antibody
    • Mitotin antibody
    • Nuclear protein BM28 antibody
    • OTTHUMP00000216047 antibody
    • OTTHUMP00000216050 antibody
    see all

Anti-MCM2 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labelling MCM2 with ab4461 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MCM2 antibody (ab4461) at 1 µg/ml

    Lane 1 : NIH3T3 whole cell lysate
    Lane 2 : TCMK-1 whole cell lysate
    Lane 3 : 4T1 whole cell lysate
    Lane 4 : CT26 whole cell lysate

    Lysates/proteins at 50 µg per lane.


    Predicted band size : 100 kDa


  • Predicted band size : 100 kDa

    Sample: HeLa cell RIPA extract.

    WB - 50 µg.

    CoIP - 7 µg.

    ab4461 incubated at 0.2 µg/ml for 1 hour for WB.

    ab4461 incubated at 20 µg for CoIP.

    Detection:

    WB -ECL

    CoIP - Coomassie Stain

    Sample: HeLa cell RIPA extract.

    WB - 50 µg.

    CoIP - 7 µg.

    ab4461 incubated at 0.2 µg/ml for 1 hour for WB.

    ab4461 incubated at 20 µg for CoIP.

    Detection:

    WB -ECL

    CoIP - Coomassie Stain
  • ICC/IF image of ab4461 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4461, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Ab4461 staining MCM2 in Human placenta.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-MCM2 antibody (ab4461)

This product has been referenced in:
  • Kirkham M  et al. Progenitor Cell Dynamics in the Newt Telencephalon during Homeostasis and Neuronal Regeneration. Stem Cell Reports 2:507-19 (2014). IHC ; Newt . Read more (PubMed: 24749074) »
  • Huang J  et al. The DNA translocase FANCM/MHF promotes replication traverse of DNA interstrand crosslinks. Mol Cell 52:434-46 (2013). Mouse . Read more (PubMed: 24207054) »

See all 8 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 20 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 01 2013

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monit...

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Application Immunocytochemistry
Sample Human Cultured Cells (Breast cancer cell line)
Specification Breast cancer cell line
Fixative Methanol
Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Rene Villadsen

Verified customer

Submitted Jul 02 2011

Application Immunoprecipitation
Sample Chicken Cell lysate - nuclear (DT40)
Total protein in input 50 µg
Specification DT40
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

Submitted Sep 28 2009

Application Western blot
Sample Xenopus laevis Cell lysate - other (Interphasic egg extract)
Loading amount 25 µg
Specification Interphasic egg extract
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Domenico Maiorano

Verified customer

Submitted Jul 17 2009

Application Immunoprecipitation
Sample Xenopus laevis Cell lysate - whole cell (egg extracts)
Total protein in input 500 µg
Specification egg extracts
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

Submitted Dec 10 2008

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Loading amount 50 µg
Specification Mouse embryonic fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 20 2007

Thanks for your enquiry. Unfortunately, we are not aware of any publications that feature the use of either of these antibodies. We also have not received any feedback from customers in the form of reviews who have used these antibodies. For both...

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All the information available for this product is listed on the on-line datasheet (price, datasheet, immunogen, publication, suitability, cross-reactivity). I suggest you take a look at the datasheets for these particular products in order to ascertain...

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