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Western blot - MCM2 antibody (ab4461)
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Immunocytochemistry/ Immunofluorescence-MCM2 antibody(ab4461)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MCM2 antibody(ab4461)
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Product Name
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MCM2 antibody
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See all MCM2 antibodies (17)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MCM2
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Immunogen
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Synthetic peptide (Human) - 37 amino acids which represent a portion of human MCM 2 encoded within exon 2.
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Reacts with
(species key)
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Hu, Ms, Chk, Xl
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Tested applications
(see key)
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ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IP: Use at a concentration of 2 - 5 µg/ml.
WB: 1/2000 - 1/10000. Predicted molecular weight: 100 kDa.
IHC-P: Use at a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Relevance
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MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. It is similar to members of the family of early S phase proteins. Mincheva et al. (1994) mapped the gene to 3q21. From its localization, CDCL1 became a candidate for an oncogene affected by chromosomal breaks in acute myeloid leukemia (AML)
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MCM2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MCM2 antibody images, references, products related to ab4461 and other tools.
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MCM2 antibody images:
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 Western blot - MCM2 antibody (ab4461)
Predicted band size : 100 kDa
Sample: HeLa cell RIPA extract.
WB - 50 µg.
CoIP - 7 µg.
ab4461 incubated at 0.2 µg/ml for 1 hour for WB.
ab4461 incubated at 20 µg for CoIP.
Detection:
WB -ECL
CoIP - Coomassie Stain
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Immunocytochemistry/ Immunofluorescence-MCM2 antibody(ab4461)
ICC/IF image of ab4461 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4461, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MCM2 antibody(ab4461)
Ab4461 staining MCM2 in Human placenta.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for MCM2 antibody (ab4461)
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This product has been used in: (two most recent references)
L vy N et al. XRCC1 interacts with the p58 subunit of DNA Pol alpha-primase and may coordinate DNA repair and replication during S phase. Nucleic Acids Res 37:3177-88 (2009). WB; Xenopus laevis. PubMed: 19305001
Rajesh C et al. The interaction profile of homologous recombination repair proteins RAD51C, RAD51D and XRCC2 as determined by proteomic analysis. Proteomics 9:4071-86 (2009). WB; Mouse. PubMed: 19658102
See all 4 publication references for this product.
If you publish research using ab4461 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MCM2
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MCM2 antibody - more information
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Customer reviews (feedback) regarding MCM2 antibody |
Customer FAQs regarding MCM2 antibody |
Protocols for MCM2 antibody |
Price and availability of products related to MCM2 antibody |
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MCM2 antibody - related products:
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MCM2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MCM2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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