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Synthetic peptide corresponding to Human MCM2. The epitope recognized maps to a region between residues 1 and 50 of human human minichromosomal maintenance deficient 2 using the numbering given in entry NP_0045117.2 (GeneID 4171).
Our Abpromise guarantee covers the use of ab4461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21068061|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 100 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|ICC||Use at an assay dependent concentration.|
Sample: HeLa cell RIPA extract.
WB - 50
CoIP - 7
ab4461 incubated at 0.2
ab4461 incubated at 20
CoIP - Coomassie StainSample: HeLa cell RIPA extract.
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