Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)

Overview

• Product nameAnti-MCM2 (phospho S108) antibody [EPR4121]See all MCM2 primary antibodies ...
• Description
  Rabbit monoclonal [EPR4121] to MCM2 (phospho S108)
• Specificityab109271 only detects MCM2 phosphorylated at serine 108.
• Tested applicationsWB, IP, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Phospho specific peptide corresponding to residues surrounding Serine 108 of Human MCM2.

• Positive controlHeLa cell lysate; HeLa cells.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR4121
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Synthesis
  • Other

Applications

Target

• FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
• Sequence similaritiesBelongs to the MCM family.
  Contains 1 MCM domain.
• Post-translational
  modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
• Cellular localizationNucleus.

Target information above from: UniProt accession P49736 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4171 Human
  • Entrez Gene: 17216 Mouse
  • Entrez Gene: 312538 Rat
  • Omim: 116945 Human
  • SwissProt: P49736 Human
  • SwissProt: P97310 Mouse
  • Unigene: 477481 Human
  • Unigene: 16711 Mouse

  • Unigene: 2715 Rat

  see all

• Alternative names
  BM28 antibodyCCNL 1 antibodyCCNL1 antibody

  CDC like 1 antibodycdc19 antibodyCDCL 1 antibodyCDCL1 antibodyCell devision cycle like 1 antibodyCyclin like 1 antibodyD3S3194 antibodyDNA replication licensing factor MCM2 antibodyKIAA0030 antibodyMCM 2 antibodyMCM2 antibodyMCM2 minichromosome maintenance deficient 2 mitotin antibodyMCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibodyMCM2 minichromosome maintenance deficient 2, mitotin antibodyMCM2_HUMAN antibodyMGC10606 antibodyMinichromosome maintenance complex component 2 antibodyMinichromosome maintenance deficient 2 (mitotin) antibodyMinichromosome maintenance deficient 2 mitotin antibodyMinichromosome maintenance protein 2 antibodyMinichromosome maintenance protein 2 homolog antibodyMitotin antibodyNuclear protein BM28 antibodyOTTHUMP00000216047 antibodyOTTHUMP00000216050 antibody

  see all

Anti-MCM2 (phospho S108) antibody [EPR4121] images

• 

  Western blot - MCM2 (phospho S108) antibody [EPR4121] (ab109271)
  All lanes : Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271) at 1/1000 dilution
  
  Lane 1 : HeLa cell lysate, untreated
  Lane 2 : HeLa cell lysate, treated with Alkaline Phosphatase (AP)
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 102 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - MCM2 (phospho S108) antibody [EPR4121] (ab109271)
  Immunofluorescent staining of HeLa cells using ab109271 at a dilution of 1/100.