Anti-MCM2 (phospho S27) antibody [EPR4119] (ab109459)

Overview

• Product nameAnti-MCM2 (phospho S27) antibody [EPR4119]See all MCM2 primary antibodies ...
• Description
  Rabbit monoclonal [EPR4119] to MCM2 (phospho S27)
• SpecificityThis antibody only detects MCM2 when phosphorylated at serine 27.
• Tested applicationsWB, IP, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A phospho specific peptide corresponding to residues surrounding serine 27 of Human MCM2.

• Positive controlHeLa cell lysate and HeLa cells. Human cervical carcinoma tissue. Human tonsil tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Store at -20ºC.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR4119
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Synthesis
  • Other

Applications

Target

• FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
• Sequence similaritiesBelongs to the MCM family.
  Contains 1 MCM domain.
• Post-translational
  modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
• Cellular localizationNucleus.

Target information above from: UniProt accession P49736 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4171 Human
  • Entrez Gene: 17216 Mouse
  • Entrez Gene: 312538 Rat
  • Omim: 116945 Human
  • SwissProt: P49736 Human
  • SwissProt: P97310 Mouse
  • Unigene: 477481 Human
  • Unigene: 16711 Mouse

  • Unigene: 2715 Rat

  see all

• Alternative names
  BM28 antibodyCCNL 1 antibodyCCNL1 antibody

  CDC like 1 antibodycdc19 antibodyCDCL 1 antibodyCDCL1 antibodyCell devision cycle like 1 antibodyCyclin like 1 antibodyD3S3194 antibodyDNA replication licensing factor MCM2 antibodyKIAA0030 antibodyMCM 2 antibodyMCM2 antibodyMCM2 minichromosome maintenance deficient 2 mitotin antibodyMCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibodyMCM2 minichromosome maintenance deficient 2, mitotin antibodyMCM2_HUMAN antibodyMGC10606 antibodyMinichromosome maintenance complex component 2 antibodyMinichromosome maintenance deficient 2 (mitotin) antibodyMinichromosome maintenance deficient 2 mitotin antibodyMinichromosome maintenance protein 2 antibodyMinichromosome maintenance protein 2 homolog antibodyMitotin antibodyNuclear protein BM28 antibodyOTTHUMP00000216047 antibodyOTTHUMP00000216050 antibody

  see all

Anti-MCM2 (phospho S27) antibody [EPR4119] images

• 

  Western blot - MCM2 (phospho S27) antibody [EPR4119] (ab109459)
  All lanes : Anti-MCM2 (phospho S27) antibody [EPR4119] (ab109459) at 1/10000 dilution
  
  Lane 1 : HeLa cell lysates untreated with alkaline phosphatase
  Lane 2 : HeLa cell lysates treated with alkaline phosphatase
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP-labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 102 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 (phospho S27) antibody [EPR4119] (ab109459)
  ab109459 at 1/100 dilution staining MCM2 (phospho S27) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 (phospho S27) antibody [EPR4119] (ab109459)
  ab109459 at 1/100 dilution staining MCM2 (phospho S27) in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
• 

  Immunocytochemistry/ Immunofluorescence - MCM2 (phospho S27) antibody [EPR4119] (ab109459)
  ab109459 at 1/100 dilution staining MCM2 (phospho S27) in HeLa cells by Immunofluorescence.