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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-MCM2 (phospho S27) antibody
See all MCM2 products (25) ...
Description
Rabbit polyclonal to MCM2 (phospho S27)
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Immunogen was a synthetic phosphorylated peptide, which represented a portion of human MCM2 surrounding Serine 18 (LocusLink ID 4171).
General notes
Centrifuge tube to remove product from lid.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
This protein is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Western blot - MCM2 (phospho S27) antibody (ab11896)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/10,000 - 1/20,000.Detects a band of approximately 125 kDa (predicted molecular weight: 108 kDa).Can be blocked with MCM2 peptide (ab40230).
Target
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain.
Post-translational
modifications
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP49736
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
Database links
- Entrez Gene: 4171 Human
- Omim: 116945 Human
- SwissProt: P49736 Human
- Unigene: 477481 Human
Anti-MCM2 (phospho S27) antibody images:
Western blot - MCM2 (phospho S27) antibody (ab11896)
All lanes : Anti-MCM2 (phospho S27) antibody (ab11896) at 0.07 µg/ml
Lane 1 : HeLa whole cell lysate.
Lane 2 : HeLa whole cell lysate with with blocking phosphopeptide.
Lysates/proteins at 30 µg per lane.
Predicted band size : 108 kDa
Exposure time : 1 minute
ab4461 was used at 0.2 µg/ml to show total MCM2.
Detection: Chemiluminescence
References for Anti-MCM2 (phospho S27) antibody (ab11896)
ab11896 has not yet been referenced specifically in any publications.
Publishing research using ab11896? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"