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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-MCM2 (phospho S27) antibody
See all MCM2 products (25) ...
Description
Rabbit polyclonal to MCM2 (phospho S27)
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic phosphorylated peptide corresponding to a region of Human MCM2 surrounding Serine 27 (NP_004517.2).
Positive control
Human colon carcinoma. Mouse squamous cell carcinoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
IHC-P
Application notes
IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain.
Post-translational
modifications
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP49736
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
Database links
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- Unigene: 477481 Human
- Unigene: 16711 Mouse
Anti-MCM2 (phospho S27) antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 (phospho S27) antibody (ab84142)
ab84142, at 1/250 dilution, staining MCM2 in formalin-fixed, paraffin-embedded serial sections of human colon carcinoma by Immunohistochemistry.
Mock phosphatase treated section (left)
CIP phosphatase treated section (right).
Detection: DAB staining using anti-Rabbit HRP conjugated antibody at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 (phospho S27) antibody (ab84142)
ab84142, at 1/250 dilution, staining MCM2 in formalin-fixed, paraffin-embedded mouse squamous cell carcinoma tissue by Immunohistochemistry using DAB detection.
References for Anti-MCM2 (phospho S27) antibody (ab84142)
ab84142 has not yet been referenced specifically in any publications.
Publishing research using ab84142? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"