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Western blot - MCM2 (phospho S32) antibody (ab11897)
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Product Name
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MCM2 (phospho S32) antibody
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See all MCM2 antibodies (17)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MCM2 (phospho S32)
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Immunogen
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Immunogen was a synthetic phosphorylated peptide, which represented a portion of human MCM2 surrounding Serine32 (LocusLink ID 4171).
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/5000 - 1/20,000. Detects a band of approximately 125 kDa (predicted molecular weight: 108 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Relevance
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MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. It is similar to members of the family of early S phase proteins. Mincheva et al. (1994) mapped the gene to 3q21. From its localization, CDCL1 became a candidate for an oncogene affected by chromosomal breaks in acute myeloid leukemia (AML)
This protein is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
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MCM2 (phospho S32) antibody (ab11897) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Centrifuge tube to remove product from lid.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MCM2 (phospho S32) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MCM2 (phospho S32) antibody images, references, products related to ab11897 and other tools.
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MCM2 (phospho S32) antibody images:
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 Western blot - MCM2 (phospho S32) antibody (ab11897)
Detection of Human Phospho MCM2 (Ser32) by Western Blot.
Predicted band size : 108 kDa
Samples: Whole cell lysate (30 µg) from HeLa cells that were either untreated or treated with alkaline phosphatase (AP). Antibodies: ab1187 used at 0.1 µg/ml. After probing with the phospho-specific antibodies, ab4461 was used at 0.2 µg/ml to show total MCM2. Detection: Chemiluminescence with a 1 minute exposure.
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References for MCM2 (phospho S32) antibody (ab11897)
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This product has been used in:
Canela N et al. Identification of an hexapeptide that binds to a surface pocket in cyclin A and inhibits the catalytic activity of the complex cyclin-dependent kinase 2-cyclin A. J Biol Chem 281:35942-53 (2006). WB; Human. PubMed: 17001081
If you publish research using ab11897 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MCM2
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MCM2 (phospho S32) antibody - more information
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