Anti-MCM2 (phospho S40 + S41) antibody (ab70371)

Overview

• Product nameAnti-MCM2 (phospho S40 + S41) antibodySee all MCM2 primary antibodies ...
• Description
  Rabbit polyclonal to MCM2 (phospho S40 + S41)
• Tested applicationsWB, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  A synthetic di-phosphorylated peptide, corresponding to a sequence from human MCM2 surrounding Serines 40 and 41. (NP_004517.2)

• Positive controlWhole cell lysate from asynchronous 293T cells

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Synthesis
  • Other

Applications

Target

• FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
• Sequence similaritiesBelongs to the MCM family.
  Contains 1 MCM domain.
• Post-translational
  modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
• Cellular localizationNucleus.

Target information above from: UniProt accession P49736 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4171 Human
  • Omim: 116945 Human
  • SwissProt: P49736 Human
  • Unigene: 477481 Human

• Alternative names
  BM28 antibodyCCNL 1 antibodyCCNL1 antibody

  CDC like 1 antibodyCDC like-1 antibodycdc19 antibodyCDCL 1 antibodyCDCL1 antibodyCell devision cycle like 1 antibodyCyclin like 1 antibodycyclin like-1 antibodyD3S3194 antibodyDNA replication licensing factor MCM2 antibodyKIAA0030 antibodyMCM 2 antibodyMCM2 antibodyMCM2 minichromosome maintenance deficient 2 mitotin antibodyMCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibodyMCM2 minichromosome maintenance deficient 2, mitotin antibodyMCM2_HUMAN antibodyMGC10606 antibodyMinichromosome maintenance complex component 2 antibodyMinichromosome maintenance deficient 2 (mitotin) antibodyMinichromosome maintenance deficient 2 mitotin antibodyMinichromosome maintenance protein 2 antibodyMinichromosome maintenance protein 2 homolog antibodyMitotin antibodyNuclear protein BM28 antibodyOTTHUMP00000216047 antibodyOTTHUMP00000216050 antibody

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Anti-MCM2 (phospho S40 + S41) antibody images

• 

  Western blot - MCM2 (phospho S40 + S41) antibody (ab70371)
  All lanes : Anti-MCM2 (phospho S40 + S41) antibody (ab70371) at 0.1 µg/ml
  
  Lane 1 : Whole cell lysate from asynchronous 293T cells
  Lane 2 : Whole cell lysate from asynchronous 293T cells treated with a phosphatase
  
  Lysates/proteins at 50 µg per lane.
  
  
  Predicted band size : 100 kDa
  Observed band size : 117 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
• 

  Flow Cytometry - MCM2 (phospho S40 + S41) antibody (ab70371)

  ab70371 at 0.1µg/ml staining MCM2 (phospho S40 + S41) by Flow Cytometery. 1x10⁶ cells were fixed in 1.5% PFA and permeabilized in 90% MeOH. DNA content was simultaneously analyzed via PI stain.