RabMAb

Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

Overview

  • Product nameAnti-MCM2 (phospho S53) antibody [EP4120]
    See all MCM2 primary antibodies
  • Description
    Rabbit monoclonal [EP4120] to MCM2 (phospho S53)
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Phospho specific peptide corresponding to residues surrounding Serine 53 of Human MCM2.

  • Positive control
    • HeLa cell lysates (untreated or treated with alkaline phosphatase (AP)) Human tonsil tissue, HeLa cells
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°C
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • Clonality Monoclonal
  • Clone numberEP4120
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab109133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 125 kDa (predicted molecular weight: 102 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
    • Sequence similaritiesBelongs to the MCM family.
      Contains 1 MCM domain.
    • Post-translational
      modifications
      Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BM28 antibody
      • CCNL 1 antibody
      • CCNL1 antibody
      • CDC like 1 antibody
      • cdc19 antibody
      • CDCL 1 antibody
      • CDCL1 antibody
      • Cell devision cycle like 1 antibody
      • Cyclin like 1 antibody
      • D3S3194 antibody
      • DNA replication licensing factor MCM2 antibody
      • KIAA0030 antibody
      • MCM 2 antibody
      • MCM2 antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
      • MCM2 minichromosome maintenance deficient 2, mitotin antibody
      • MCM2_HUMAN antibody
      • MGC10606 antibody
      • Minichromosome maintenance complex component 2 antibody
      • Minichromosome maintenance deficient 2 (mitotin) antibody
      • Minichromosome maintenance deficient 2 mitotin antibody
      • Minichromosome maintenance protein 2 antibody
      • Minichromosome maintenance protein 2 homolog antibody
      • Mitotin antibody
      • Nuclear protein BM28 antibody
      • OTTHUMP00000216047 antibody
      • OTTHUMP00000216050 antibody
      see all

    Anti-MCM2 (phospho S53) antibody [EP4120] images

    • All lanes : Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133) at 1/10000 dilution

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate treated with alkaline phosphatase (AP)

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 102 kDa
    • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using ab109133 at a dilution of 1/100.
    • Immunofluorescent staining of HeLa cells using ab109133 at a dilution of 1/50.

    References for Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

    ab109133 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293T)
    Loading amount 50 µg
    Specification HEK293T
    Treatment Cdc7 RNAi
    Gel Running Conditions Reduced Denaturing (8% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Feb 27 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"