Overview

  • Product nameAnti-MCM2 (phospho S53) antibody
    See all MCM2 primary antibodies
  • Description
    Rabbit polyclonal to MCM2 (phospho S53)
  • Tested applicationsIHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic phosphorylated peptide corresponding to a portion of human MCM2 (NP_004517.2) surrounding serine 53.

  • Positive control
    • Human controls: breast carcinoma, colon adenocarcinoma, ovarian carcinoma, stomach adenocarcinoma and testicular seminoma. Mouse controls: squamous cell carcinoma.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab83969 was affinity purified using an epitope specific to MCM2 (phospho S53) immobilized on a solid support.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab83969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500.
    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
    • Sequence similaritiesBelongs to the MCM family.
      Contains 1 MCM domain.
    • Post-translational
      modifications
      Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BM28 antibody
      • CCNL 1 antibody
      • CCNL1 antibody
      • CDC like 1 antibody
      • CDC like-1 antibody
      • cdc19 antibody
      • CDCL 1 antibody
      • CDCL1 antibody
      • Cell devision cycle like 1 antibody
      • Cyclin like 1 antibody
      • cyclin like-1 antibody
      • D3S3194 antibody
      • DNA replication licensing factor MCM2 antibody
      • KIAA0030 antibody
      • MCM 2 antibody
      • MCM2 antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
      • MCM2 minichromosome maintenance deficient 2, mitotin antibody
      • MCM2_HUMAN antibody
      • MGC10606 antibody
      • Minichromosome maintenance complex component 2 antibody
      • Minichromosome maintenance deficient 2 (mitotin) antibody
      • Minichromosome maintenance deficient 2 mitotin antibody
      • Minichromosome maintenance protein 2 antibody
      • Minichromosome maintenance protein 2 homolog antibody
      • Mitotin antibody
      • Nuclear protein BM28 antibody
      • OTTHUMP00000216047 antibody
      • OTTHUMP00000216050 antibody
      see all

    Anti-MCM2 (phospho S53) antibody images

    • ab83969, at a 1/500 dilution, staining MCM2 (phospho S53) in formaldehyde fixed, paraffin embedded human colon adenocarcinoma sections by Immunohistochemistry.
      Left image: mock phosphatase treated section
      Right image: calf intestinal phosphatase treated section.
      Detection: DAB staining using anti-rabbit IHC antibody at a dilution of 1/100.
    • ab83969, at a 1/500 dilution, staining MCM2 (phospho S53) in formaldehyde fixed, paraffin embedded mouse squamous cell carcinoma sections by Immunohistochemistry.
      Left image: mock phosphatase treated section
      Right image: calf intestinal phosphatase treated section.
      Detection: DAB staining using anti-rabbit IHC antibody at a dilution of 1/100.

    References for Anti-MCM2 (phospho S53) antibody (ab83969)

    ab83969 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"