Anti-MCM2 (phospho S53) antibody (ab83969)

Overview

• Product nameAnti-MCM2 (phospho S53) antibodySee all MCM2 primary antibodies ...
• Description
  Rabbit polyclonal to MCM2 (phospho S53)
• Tested applicationsIHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic phosphorylated peptide corresponding to a portion of human MCM2 (NP_004517.2) surrounding serine 53.

• Positive controlHuman controls: breast carcinoma, colon adenocarcinoma, ovarian carcinoma, stomach adenocarcinoma and testicular seminoma. Mouse controls: squamous cell carcinoma.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab83969 was affinity purified using an epitope specific to MCM2 (phospho S53) immobilized on a solid support.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Synthesis
  • Other

Applications

Target

• FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
• Sequence similaritiesBelongs to the MCM family.
  Contains 1 MCM domain.
• Post-translational
  modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
• Cellular localizationNucleus.

Target information above from: UniProt accession P49736 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4171 Human
  • Entrez Gene: 17216 Mouse
  • Omim: 116945 Human
  • SwissProt: P49736 Human
  • SwissProt: P97310 Mouse
  • Unigene: 477481 Human
  • Unigene: 16711 Mouse

• Alternative names
  BM28 antibodyCCNL 1 antibodyCCNL1 antibody

  CDC like 1 antibodyCDC like-1 antibodycdc19 antibodyCDCL 1 antibodyCDCL1 antibodyCell devision cycle like 1 antibodyCyclin like 1 antibodycyclin like-1 antibodyD3S3194 antibodyDNA replication licensing factor MCM2 antibodyKIAA0030 antibodyMCM 2 antibodyMCM2 antibodyMCM2 minichromosome maintenance deficient 2 mitotin antibodyMCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibodyMCM2 minichromosome maintenance deficient 2, mitotin antibodyMCM2_HUMAN antibodyMGC10606 antibodyMinichromosome maintenance complex component 2 antibodyMinichromosome maintenance deficient 2 (mitotin) antibodyMinichromosome maintenance deficient 2 mitotin antibodyMinichromosome maintenance protein 2 antibodyMinichromosome maintenance protein 2 homolog antibodyMitotin antibodyNuclear protein BM28 antibodyOTTHUMP00000216047 antibodyOTTHUMP00000216050 antibody

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Anti-MCM2 (phospho S53) antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 (phospho S53) antibody (ab83969)
  ab83969, at a 1/500 dilution, staining MCM2 (phospho S53) in formaldehyde fixed, paraffin embedded human colon adenocarcinoma sections by Immunohistochemistry.
  Left image: mock phosphatase treated section
  Right image: calf intestinal phosphatase treated section.
  Detection: DAB staining using anti-rabbit IHC antibody at a dilution of 1/100.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 (phospho S53) antibody (ab83969)
  ab83969, at a 1/500 dilution, staining MCM2 (phospho S53) in formaldehyde fixed, paraffin embedded mouse squamous cell carcinoma sections by Immunohistochemistry.
  Left image: mock phosphatase treated section
  Right image: calf intestinal phosphatase treated section.
  Detection: DAB staining using anti-rabbit IHC antibody at a dilution of 1/100.