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ab4460 has been referenced in 9 publications.
Publishing research using ab4460? Please let us know so that we can cite the reference in this datasheet
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Predicted band size : 90 kDa
HeLa cell RIPA extract (50 µg) incubated with ab4460 used at 0.2 µg/ml for 1 hour.
Predicted band size : 90 kDa
Western blot analysis of ab4460 on HeLa OHIO extract.
ICC/IF image of ab4460 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4460, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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