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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-MCM3 antibody - ChIP Grade
See all MCM3 products (8) ...
Description
Rabbit polyclonal to MCM3 - ChIP Grade
Tested applications
ICC/IF, IP, WB, ChIPmore details
Cross reactivity
Reacts with
Chicken, Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide (Human) - 38 amino acids which represent a portion of human MCM 3 encoded in part by exons 10 and 11.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
IP: Use a concentration of 2 - 5 µg/ml.
WB: 1/2000 - 1/10000.Detects a band of approximately 105 kDa (predicted molecular weight: 90 kDa).
ChIP: Use at an assay dependent dilution. (PubMed: 19073720)
Target
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP25205
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cervical cancer proto oncogene 5 antibody
- DNA polymerase alpha holoenzyme associated P1 antibody
- DNA polymerase alpha holoenzyme associated protein P1 antibody
see all
Database links
- Entrez Gene: 4172 Human
- Entrez Gene: 17215 Mouse
- Omim: 602693 Human
- SwissProt: P25205 Human
- SwissProt: P25206 Mouse
- Unigene: 179565 Human
- Unigene: 4502 Mouse
Anti-MCM3 antibody - ChIP Grade images:
Western blot - Anti-MCM3 antibody - ChIP Grade (ab4460)
Predicted band size : 90 kDa
HeLa cell RIPA extract (50 µg) incubated with ab4460 used at 0.2 µg/ml for 1 hour.
Western blot - MCM3 antibody (ab4460)
Predicted band size : 90 kDa
Western blot analysis of ab4460 on HeLa OHIO extract.
Immunocytochemistry/ Immunofluorescence-MCM3 antibody - ChIP Grade(ab4460)
ICC/IF image of ab4460 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4460, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-MCM3 antibody - ChIP Grade (ab4460)
This product has been referenced in:
- Fragkos M & Beard P Mitotic Catastrophe Occurs in the Absence of Apoptosis in p53-Null Cells with a Defective G1 Checkpoint. PLoS One 6:e22946 (2011). WB; Human.Read more (PubMed: 21853057) »
- Yasuda Aet al. DNA ligand designed to antagonize EBNA1 represses Epstein-Barr virus-induced immortalization. Cancer Sci : (2011). IP.Read more (PubMed: 21910783) »
See all 9 publications for this product
Publishing research using ab4460? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"