Recombinant Anti-MCM3 antibody [EPR7080] (ab128923)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7080] to MCM3
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MCM3 antibody [EPR7080]
See all MCM3 primary antibodies -
Description
Rabbit monoclonal [EPR7080] to MCM3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- L6, C6, RAW 264.7, PC-12, NIH/3T3, HeLa, K562, HepG2, U937 and 293T cell lysates. Human colon and tonsil tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7080 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4172 Human
- Entrez Gene: 17215 Mouse
- Entrez Gene: 316273 Rat
- Omim: 602693 Human
- SwissProt: P25205 Human
- SwissProt: P25206 Mouse
- Unigene: 179565 Human
- Unigene: 4502 Mouse
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Alternative names
- Cervical cancer proto oncogene 5 antibody
- DNA polymerase alpha holoenzyme associated P1 antibody
- DNA polymerase alpha holoenzyme associated protein P1 antibody
see all
Images
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All lanes : Anti-MCM3 antibody [EPR7080] (ab128923) at 1/1000 dilution
Lane 1 : L6 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : RAW 264.7 cell lysate
Lane 4 : PC-12 cell lysate
Lane 5 : NIH/3T3 cell lysate
Lane 6 : HeLa cell lysate
Lane 7 : K562 cell lysate
Lane 8 : HepG2 cell lysate
Lane 9 : U937 cell lysate
Lane 10 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 91 kDa -
ab128923 showing positive staining in Normal colon tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab128923 at 1/100 dilution staining MCM3 in paraffin-embedded Human colon tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab128923 at 1/100 dilution staining MCM3 in paraffin-embedded Human tonsil tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab128923 showing positive staining in Breast carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab128923 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab128923 showing positive staining in Lung carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling MCM3 with unpurified ab128923 at 1/900 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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ab128923 showing positive staining in Cervical carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab128923 has been referenced in 6 publications.
- Lei Y et al. Identification of MCM family as potential therapeutic and prognostic targets for hepatocellular carcinoma based on bioinformatics and experiments. Life Sci 272:119227 (2021). PubMed: 33607151
- Cao T et al. Identification of the DNA Replication Regulator MCM Complex Expression and Prognostic Significance in Hepatic Carcinoma. Biomed Res Int 2020:3574261 (2020). PubMed: 32964028
- Li HT et al. Diagnostic and prognostic value of MCM3 and its interacting proteins in hepatocellular carcinoma. Oncol Lett 20:308 (2020). PubMed: 33093917
- Niu Y et al. Huaier Suppresses the Hepatocellular Carcinoma Cell Cycle by Regulating Minichromosome Maintenance Proteins. Onco Targets Ther 13:12015-12025 (2020). PubMed: 33244243
- Zhang K et al. Demethylzeylasteral inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axis. Cell Death Dis 9:1035 (2018). PubMed: 30305611
- Ye Y et al. Up-regulation of REG3A in colorectal cancer cells confers proliferation and correlates with colorectal cancer risk. Oncotarget 7:3921-33 (2016). WB . PubMed: 26646797