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Western blot - MCM3 antibody (ab3724)
(enlarge)
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Product Name
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MCM3 antibody
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See all MCM3 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to MCM3
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Immunogen
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Synthetic peptide (Human) - which represents a portion of human Minichromosome Maintenance Deficient 3 encoded in part by exons 1 and 2.
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/2000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Relevance
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MCM3 is one of the highly conserved mini-chromosome maintenance proteins that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MCM3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MCM3 antibody images, references, products related to ab3724 and other tools.
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MCM3 antibody images:
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 Western blot - MCM3 antibody (ab3724)
Predicted band size : 110 kDa
Sample: Hela cell RIPA extract (60µg). Antibody: Affinity purified Goat anti-MCM 3 (ab3724) diluted 1 / 4000. Detection: ECL.
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Search PubMed (MEDLINE) for references to MCM3
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MCM3 antibody - more information
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Customer reviews (feedback) regarding MCM3 antibody |
Customer FAQs regarding MCM3 antibody |
Protocols for MCM3 antibody |
Price and availability of products related to MCM3 antibody |
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MCM3 antibody - related products:
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MCM3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MCM3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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