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Anti-MCM3 antibody (ab60597) + HeLa cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
developed using the ECL technique
Predicted band size : 90 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
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