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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-MCM3 antibody
See all MCM3 products (8) ...
Description
Mouse monoclonal to MCM3
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Cow
Immunogen
Recombinant fragment with tag: AKDGDSYDPY DFSDTEEEMP QVHTPKTADS QETKESQKVE LSESRLKAFK VALLDVFREA HAQSIGMNRL TESINRDSEE PFSSVEIQAA LSKMQDDNQV MVSEGIIFLI , corresponding to amino acids 699-809 of Human MCM3
AKDGDSYDPY DFSDTEEEMP QVHTPKTADS QETKESQKVE LSESRLKAFK VALLDVFREA HAQSIGMNRL TESINRDSEE PFSSVEIQAA LSKMQDDNQV MVSEGIIFLI
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2b
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Western blot - MCM3 antibody (ab60597)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab60597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 100 kDa (predicted molecular weight: 90 kDa).
Target
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP25205
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cervical cancer proto oncogene 5 antibody
- DNA polymerase alpha holoenzyme associated P1 antibody
- DNA polymerase alpha holoenzyme associated protein P1 antibody
see all
Database links
- Entrez Gene: 281302 Cow
- Entrez Gene: 4172 Human
- Omim: 602693 Human
- SwissProt: A4FUD9 Cow
- SwissProt: P25205 Human
- Unigene: 179565 Human
Anti-MCM3 antibody images:
Western blot - MCM3 antibody (ab60597)
Anti-MCM3 antibody (ab60597) + HeLa cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
developed using the ECL technique
Predicted band size : 90 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
References for Anti-MCM3 antibody (ab60597)
This product has been referenced in:
- Ferguson RLet al. The cyclin A centrosomal localization sequence recruits MCM5 and Orc1 to regulate centrosome reduplication. J Cell Sci 123:2743-9 (2010). WB; Human.Read more (PubMed: 20663915) »
See 1 publication for this product
Publishing research using ab60597? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"