Anti-MCM3 antibody (ab80456)

Overview

• Product nameAnti-MCM3 antibodySee all MCM3 primary antibodies ...
• Description
  Rabbit polyclonal to MCM3
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow
• Immunogen

  Synthetic peptide: LVQYAYFKKV LEKEKKRKKR SEDESETEDE EEKSQEDQEQ KRKRRKTRQP , corresponding to C terminal amino acids 649-698 of Human MCM3

  Run BLAST with Run BLAST with
• Positive control721_B cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Synthesis
  • Other

Applications

Target

• FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
• Sequence similaritiesBelongs to the MCM family.
  Contains 1 MCM domain.
• Post-translational
  modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
• Cellular localizationNucleus.

Target information above from: UniProt accession P25205 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 281302 Cow
  • Entrez Gene: 4172 Human
  • Entrez Gene: 17215 Mouse
  • Entrez Gene: 316273 Rat
  • Omim: 602693 Human
  • SwissProt: A4FUD9 Cow
  • SwissProt: P25205 Human
  • SwissProt: P25206 Mouse

  • Unigene: 179565 Human
  • Unigene: 4502 Mouse

  see all

• Alternative names
  Cervical cancer proto oncogene 5 antibodyDNA polymerase alpha holoenzyme associated P1 antibodyDNA polymerase alpha holoenzyme associated protein P1 antibody

  DNA polymerase alpha holoenzyme-associated protein P1 antibodyDNA replication factor MCM3 antibodyDNA replication licensing factor MCM3 antibodyHCC 5 antibodyHCC5 antibodyhRlf beta subunit antibodyHuman cervical cancer proto oncogene 5 antibodyMCM 3 antibodymcm3 antibodyMCM3 minichromosome maintenance deficient 3 antibodyMCM3_HUMAN antibodyMGC1157 antibodyMinichromosome maintenance complex component 3 antibodyMinichromosome maintenance deficient 3 antibodyMinichromosome maintenance protein 3 antibodyP1 h antibodyP1 MCM3 antibodyP1 Protein antibodyP1-MCM3 antibodyP1.h antibodyp102 antibodyP102 protein antibodyReplication licensing factor beta subunit antibodyRLF beta subunit antibodyRLF subunit beta antibodyRLFB antibody

  see all

Anti-MCM3 antibody images

• 

  Western blot - MCM3 antibody (ab80456)
  Anti-MCM3 antibody (ab80456) at 1 µg/ml + 721_B cell lysate at 10 µg
  
  Secondary
  HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 90 kDa
  Observed band size : 90 kDa
  Additional bands at : 39 kDa,55 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-MCM3 antibody(ab80456)
  Immunohistochemistry of Brain, cortex tissue at an antibody concentration of 5µg/ml using anti-MCM3 antibody (ab80456)