Overview

  • Product nameAnti-MCM3 antibodySee all MCM3 primary antibodies ...
  • Description
    Rabbit polyclonal to MCM3
  • Tested applicationsIHC-P, WB, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: LVQYAYFKKV LEKEKKRKKR SEDESETEDE EEKSQEDQEQ KRKRRKTRQP , corresponding to C terminal amino acids 649-698 of Human MCM3

  • Positive control
    • 721_B cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab80456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39, 55, 90 kDa (predicted molecular weight: 90 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

Target

  • FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
  • Sequence similaritiesBelongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession P25205 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Cervical cancer proto oncogene 5 antibody
    • DNA polymerase alpha holoenzyme associated P1 antibody
    • DNA polymerase alpha holoenzyme associated protein P1 antibody
    • DNA polymerase alpha holoenzyme-associated protein P1 antibody
    • DNA replication factor MCM3 antibody
    • DNA replication licensing factor MCM3 antibody
    • HCC 5 antibody
    • HCC5 antibody
    • hRlf beta subunit antibody
    • Human cervical cancer proto oncogene 5 antibody
    • MCM 3 antibody
    • mcm3 antibody
    • MCM3 minichromosome maintenance deficient 3 antibody
    • MCM3_HUMAN antibody
    • MGC1157 antibody
    • Minichromosome maintenance complex component 3 antibody
    • Minichromosome maintenance deficient 3 antibody
    • Minichromosome maintenance protein 3 antibody
    • P1 h antibody
    • P1 MCM3 antibody
    • P1 Protein antibody
    • P1-MCM3 antibody
    • P1.h antibody
    • p102 antibody
    • P102 protein antibody
    • Replication licensing factor beta subunit antibody
    • RLF beta subunit antibody
    • RLF subunit beta antibody
    • RLFB antibody
    see all

Anti-MCM3 antibody images

  • Anti-MCM3 antibody (ab80456) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 90 kDa
    Observed band size : 90 kDa
    Additional bands at : 39 kDa,55 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry of Brain, cortex tissue at an antibody concentration of 5µg/ml using anti-MCM3 antibody (ab80456)

References for Anti-MCM3 antibody (ab80456)

ab80456 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"