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Synthetic peptide: LVQYAYFKKV LEKEKKRKKR SEDESETEDE EEKSQEDQEQ KRKRRKTRQP , corresponding to C terminal amino acids 649-698 of Human MCM3
Our Abpromise guarantee covers the use of ab80456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 39, 55, 90 kDa (predicted molecular weight: 90 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/312500.
ab80456 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"