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Overview

  • Product nameMCM3 protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 182-808 of Human MCM3 with an N-terminal His tag; Predicted MWt 71kDa (UniProt P25205).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 84 µl aqua dest
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesNPLETEYGLSVYKDHQTITIQEMPEKAPAGQLPRSVDVIL DDDLVDKAKP GDRVQVVGTYRCLPGKKGGYTSGTFRTV LIACNVKQMSKDAQPSFSAEDI AKIKKFSKTRSKDIFD QLAKSLAPSIHGHDYVKKAILCLLLGGVERDLEN GSHI RGDINILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSG VGLTAA VTTDQETGERRLEAGAMVLADRGVVCIDEFDK MSDMDRTAIHEVMEQGRV TIAKAGIHARLNARCSVLAA ANPVYGRYDQYKTPMENIGLQDSLLSRFDL LFIMLDQM DPEQDREISDHVLRMHRYRAPGEQDGDAMPLGSAVDILAT DD PNFSQEDQQDTQIYEKHDNLLHGTKKKKEKMVSAAF MKKYIHVAKIIKPV LTQESATYIAEEYSRLRSQDSMSS DTARTSPVTARTLETLIRLATAHAKA RMSKTVDLQDAE EAVELVQYAYFKKVLEKEKKRKKRSEDESETEDEEEKS QEDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEEMPQVH TPKTADSQET KESQKVELSESRLKAFKVALLDVFREAH AQSIGMNRLTESINRDSEEPFS SVEIQAALSKMQDDNQ VMVSEGIIFLI
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91701 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Cervical cancer proto oncogene 5DNA polymerase alpha holoenzyme associated P1DNA polymerase alpha holoenzyme associated protein P1
        DNA polymerase alpha holoenzyme-associated protein P1DNA replication factor MCM3DNA replication licensing factor MCM3HCC 5HCC5hRlf beta subunitHuman cervical cancer proto oncogene 5MCM 3mcm3MCM3 minichromosome maintenance deficient 3MCM3_HUMANMGC1157Minichromosome maintenance complex component 3Minichromosome maintenance deficient 3Minichromosome maintenance protein 3P1 hP1 MCM3P1 ProteinP1-MCM3P1.hp102P102 proteinReplication licensing factor beta subunitRLF beta subunitRLF subunit betaRLFB
      see all
  • FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
  • Sequence similaritiesBelongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession P25205 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    MCM3 protein (Tagged-His Tag) images

    • SDS-PAGE - MCM3 protein (ab91701)
      SDS-PAGE - MCM3 protein (ab91701)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for MCM3 protein (Tagged-His Tag) (ab91701)

    ab91701 has not yet been referenced specifically in any publications.

    Publishing research using ab91701 ? Please let us know so that we can cite the reference in this datasheet

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