Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-MCM4 antibody
See all MCM4 products (9) ...
Description
Goat polyclonal to MCM4
Tested applications
Cross reactivity
Reacts with
Chicken, Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 4 encoded in part by exons 8 and 9.
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
-
Western blot - MCM4 antibody (ab3728)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
Target
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP33991
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 19G antibody
- AI325074 antibody
- AU045576 antibody
see all
Database links
- Entrez Gene: 426764 Chicken
- Entrez Gene: 4173 Human
- Entrez Gene: 17217 Mouse
- Omim: 602638 Human
- SwissProt: P33991 Human
- SwissProt: P49717 Mouse
- Unigene: 460184 Human
- Unigene: 1500 Mouse
Anti-MCM4 antibody images:
Western blot - MCM4 antibody (ab3728)
Predicted band size : 100 kDa
Sample: Hela cell RIPA extract (60
Antibody: Affinity purified Goat anti-MCM 4 (ab3728) diluted 1 / 4000.
Detection: ECL.
References for Anti-MCM4 antibody (ab3728)
This product has been referenced in:
- Tenca P et al. Cdc7 is an active kinase in human cancer cells undergoing replication stress. J Biol Chem 282:208-15 (2007).Read more (PubMed: 17062569) »
- Hochegger H et al. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol 178:257-68 (2007). WB; Human, Chicken.Read more (PubMed: 17635936) »
See all 2 publications for this product
Publishing research using ab3728? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"