![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM5 antibody [CRCT5.1 (A2.7A3)] (ab6154) image](#ab6154_1.jpg)
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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-MCM5 antibody [CRCT5.1 (A2.7A3)]
See all MCM5 products (9) ...
Description
Mouse monoclonal [CRCT5.1 (A2.7A3)] to MCM5
Specificity
Recognises human Mcm-5
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Bacterially expressed human Mcm5
Positive control
Cervical Carcinoma Tissue
General notes
Clone Number: CRCT5.1 (A2.7A3)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.1% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Clonality
Monoclonal
Clone number
CRCT5.1 (A2.7A3)
Myeloma
Sp2
Isotype
IgG2b
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM5 antibody [CRCT5.1 (A2.7A3)] (ab6154)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM5 antibody [CRCT5.1 (A2.7A3)] (ab6154) image](/ps/datasheet/Images/6/ab6154/ab6154_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab6154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 82 kDa.
IHC-P: 1/25 - 1/100.Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF: Use at an assay dependent dilution. (PubMed: 18693245)
Target
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP33992
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CDC 46 antibody
- CDC46 antibody
- CDC46 homolog antibody
see all
Database links
- Entrez Gene: 4174 Human
- Entrez Gene: 17218 Mouse
- Omim: 602696 Human
- SwissProt: P33992 Human
- SwissProt: P49718 Mouse
- Unigene: 517582 Human
- Unigene: 5048 Mouse
Anti-MCM5 antibody [CRCT5.1 (A2.7A3)] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM5 antibody [CRCT5.1 (A2.7A3)] (ab6154)
ab6154 staining MCM5 in human tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
References for Anti-MCM5 antibody [CRCT5.1 (A2.7A3)] (ab6154)
This product has been referenced in:
- Rakotomalala Let al. Hepatitis B virus X protein increases the Cdt1-to-geminin ratio inducing DNA re-replication and polyploidy. J Biol Chem 283:28729-40 (2008). ICC/IF; Mouse.Read more (PubMed: 18693245) »
- Williams GHet al. Improved cervical smear assessment using antibodies against proteins that regulate DNA replication. Proc Natl Acad Sci U S A 95:14932-7 (1998).Read more (PubMed: 9843993) »
See all 2 publications for this product
Publishing research using ab6154? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"