MCM5 antibody [EP2683Y] (ab75975)

Product Name 

MCM5 antibody [EP2683Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP2683Y] to MCM5

Immunogen 

A synthetic peptide corresponding to residues near the C terminal of human MCM5

Reacts with 

Hu, Ms

Does not react with 
(species key)

Rat

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/40.
ICC/IF: 1/100 - 1/250.
IHC-P: 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: 1/50.
WB: 1/2000 - 1/10,000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa and MCF7 cell lysates; human squamous cell cervical carcinoma tissue; HeLa cells.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other

Relevance 

MCM5 (Mini Chromosome Maintainance protein-5) is involved in the
control of DNA replication. MCM proteins have DNA dependent
ATPase motifs in their central domain which is conserved from yeast
to mammals. MCM proteins form a complex which exhibits ATPase
and helicase activities. In G0 phase levels of MCM 2 and 5 are lower
than that of MCM7 and 3 but increase as the cell enters G1/S phase of
the cell cycle.
It has been reported that immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP2683Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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