![Western blot - MCM5 antibody [EP2683Y] (ab75975) image](#ab75975wb.gif)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM5 antibody [EP2683Y] (ab75975) image](#ab75975ihc.gif)
![Immunocytochemistry/ Immunofluorescence - MCM5 antibody [EP2683Y] (ab75975) image](#ab75975if.gif)
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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-MCM5 antibody [EP2683Y]
See all MCM5 products (9) ...
Description
Rabbit monoclonal [EP2683Y] to MCM5
Tested applications
WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Does not react with
Rat
Immunogen
A synthetic peptide corresponding to residues near the C terminal of human MCM5
Positive control
HeLa and MCF7 cell lysates; human squamous cell cervical carcinoma tissue; HeLa cells.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP2683Y
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10,000.Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IP
IP: 1/50IP: 1/50
IHC-P: 1/250 - 1/500.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt
Flow Cyt: 1/40Flow Cyt: 1/40
ICC/IF: 1/100 - 1/250.
Target
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP33992
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CDC 46 antibody
- CDC46 antibody
- CDC46 homolog antibody
see all
Database links
- Entrez Gene: 4174 Human
- Entrez Gene: 17218 Mouse
- Omim: 602696 Human
- SwissProt: P33992 Human
- SwissProt: P49718 Mouse
- Unigene: 517582 Human
- Unigene: 5048 Mouse
Anti-MCM5 antibody [EP2683Y] images:
Western blot - MCM5 antibody [EP2683Y] (ab75975)
![Western blot - MCM5 antibody [EP2683Y] (ab75975)](/ps/datasheet/Images/75/ab75975/ab75975wb.gif)
All lanes : Anti-MCM5 antibody [EP2683Y] (ab75975) at 1/10000 dilution
Lane 1 : HeLa lysate
Lane 2 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 82 kDa
Observed band size : 82 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM5 antibody [EP2683Y] (ab75975)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM5 antibody [EP2683Y] (ab75975)](/ps/datasheet/Images/75/ab75975/ab75975ihc.gif)
ab75975 at 1/250 dilution staining MCM5 in human squamous cell cervical carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - MCM5 antibody [EP2683Y] (ab75975)
![Immunocytochemistry/ Immunofluorescence - MCM5 antibody [EP2683Y] (ab75975)](/ps/datasheet/Images/75/ab75975/ab75975if.gif)
ab75975 at 1/100 dilution staining MCM5 in HeLa cells by Immunofluorescence.
References for Anti-MCM5 antibody [EP2683Y] (ab75975)
ab75975 has not yet been referenced specifically in any publications.
Publishing research using ab75975? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"