|
|
|
|
- MCM5 antibody (ab17967)
(enlarge)
|
|
|
|
|
Product Name
|
MCM5 antibody
|
| |
See all MCM5 antibodies (8)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to MCM5
|
|
Immunogen
|
Synthetic peptide representing a portion of human minichromosome maintenance deficient 5 encoded within exon 2.
|
|
Reacts with
(species key)
|
Hu, Xl
|
|
Tested applications
(see key)
|
IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IP: Use at a concentration of 2 - 4 µg/mg. This antibody is efficient at pulling down the MCM2-7 complex.
WB: 1/5000 - 1/20000. Detects a band of approximately 97 kDa (predicted molecular weight: 82 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
HeLa cells.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
|
|
Relevance
|
MCM5 (Mini Chromosome Maintainance protein-5) is involved in the
control of DNA replication. MCM proteins have DNA dependent
ATPase motifs in their central domain which is conserved from yeast
to mammals. MCM proteins form a complex which exhibits ATPase
and helicase activities. In G0 phase levels of MCM 2 and 5 are lower
than that of MCM7 and 3 but increase as the cell enters G1/S phase of
the cell cycle.
It has been reported that immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MCM5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for MCM5 antibody images, references, products related to ab17967 and other tools.
|
| |
MCM5 antibody images:
|
 - MCM5 antibody (ab17967)
ab17967, at 0.2µg/ml, staining human MCM5 in (3.8, 7.5 or 15µg for lanes 1, 2 and 3 respectively) HeLa cell lysate by Western blot. ECL detection with 1 minute exposure.
|
| |
References for MCM5 antibody (ab17967)
|
|
This product has been used in: (two most recent references)
Ferguson RL & Maller JL Cyclin E-dependent localization of MCM5 regulates centrosome duplication. J Cell Sci 121:3224-32 (2008). WB; Human. PubMed: 18799789
Goetz SC et al. TBX5 is required for embryonic cardiac cell cycle progression. Development 133:2575-84 (2006). PubMed: 16728474
If you publish research using ab17967 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to MCM5
|
| |
MCM5 antibody - more information
|
Customer reviews (feedback) regarding MCM5 antibody |
Customer FAQs regarding MCM5 antibody |
Protocols for MCM5 antibody |
Price and availability of products related to MCM5 antibody |
| |
MCM5 antibody - related products:
|
|
|
| |
MCM5 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about MCM5 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
