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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-MCM5 antibody
See all MCM5 products (9) ...
Description
Rabbit polyclonal to MCM5
Tested applications
WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human, Xenopus laevis
Immunogen
Synthetic peptide representing a portion of human minichromosome maintenance deficient 5 encoded within exon 2.
Positive control
HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab17967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 - 1/20000.Detects a band of approximately 97 kDa (predicted molecular weight: 82 kDa).
IP: Use at 2-4 µg/mg of lysate. (This antibody is efficient at pulling down the MCM2-7 complex. )
ICC/IF: Use a concentration of 5 µg/ml
Target
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP33992
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CDC 46 antibody
- CDC46 antibody
- CDC46 homolog antibody
see all
Database links
- Entrez Gene: 4174 Human
- Entrez Gene: 17218 Mouse
- Entrez Gene: 380587 Xenopus laevis
- Omim: 602696 Human
- SwissProt: P33992 Human
- SwissProt: P49718 Mouse
- SwissProt: P55862 Xenopus laevis
- Unigene: 517582 Human
see all
Anti-MCM5 antibody images:
- MCM5 antibody (ab17967)
ab17967, at 0.2
Immunocytochemistry/ Immunofluorescence-MCM5 antibody(ab17967)
ICC/IF image of ab17967 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17969, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-MCM5 antibody (ab17967)
This product has been referenced in:
- Nishiyama Aet al. MCM-BP regulates unloading of the MCM2-7 helicase in late S phase. Genes Dev 25:165-75 (2011). WB; Xenopus laevis.Read more (PubMed: 21196493) »
- Afanasyeva EAet al. MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits proliferation and survival. Cell Death Differ 18:974-84 (2011).Read more (PubMed: 21233845) »
See all 6 publications for this product
Publishing research using ab17967? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"