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Overview

  • Product nameMCM5 protein (Tagged-His Tag)See all MCM5 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 217-734 of Human MCM5 with N terminal His tag; 518 amino acids, 63kDa (57.8kDa predicted from sequence). UniProt ID = P33992.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 57 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesDKCKCVDFQTLKLQELPDAVPHGEMPRHMQLYCDRYLCDK VVPGNRVTIMGIYSI KKFGLTTSRGRDRVGVGIRSSYI RVLGIQVDTDGSGRSFAGAVSPQEEEEFRRLA ALPNVY EVISKSIAPSIFGGTDMKKAIACLLFGGSRKRLPDGLTRR GDINLLMLG DPGTAKSQLLKFVEKCSPIGVYTSGKGSS AAGLTASVMRDPSSRNFIMEGGAMVL ADGGVVCIDEFD KMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAA NSV FGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNE ERDVMLAKHVITLHVSALTQ TQAVEGEIDLAKLKKFIA YCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDSD R RSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALR LFQVSTLDAALSGT LSGVEGFTSQEDQEMLSRIEKQLK RRFAIGSQVSEHSIIKDFTKQKYPEHAIHKV LQLMLRR GEIQHRMQRKVLYRLK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91935 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CDC 46CDC46CDC46 homolog
        Cell division cycle 46DNA replication licensing factorDNA replication licensing factor MCM 5DNA replication licensing factor MCM5MCM 5MCM5MCM5_HUMANMGC5315Minichromosome maintenance complex component 5Minichromosome maintenance deficient (S. cerevisiae) 5Minichromosome maintenance deficient 5Minichromosome maintenance deficient protein 5P1 CDC46P1-CDC46
      see all
  • Sequence similaritiesBelongs to the MCM family.
    Contains 1 MCM domain.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession P33992 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    MCM5 protein (Tagged-His Tag) images

    • SDS-PAGE - MCM5 protein (Tagged-His Tag) (ab91935)
      SDS-PAGE - MCM5 protein (Tagged-His Tag) (ab91935)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for MCM5 protein (Tagged-His Tag) (ab91935)

    ab91935 has not yet been referenced specifically in any publications.

    Publishing research using ab91935 ? Please let us know so that we can cite the reference in this datasheet

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